+Open data
-Basic information
Entry | Database: PDB / ID: 3ewe | ||||||
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Title | Crystal Structure of the Nup85/Seh1 Complex | ||||||
Components |
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Keywords | PROTEIN TRANSPORT / STRUCTURAL PROTEIN / Nucleoporin / Nuclear Pore Complex / mRNA transport / Nucleus / Translocation / Cell membrane / Membrane / Phosphoprotein / Transport / WD repeat / MEMBRANE PROTEIN | ||||||
Function / homology | Function and homology information Seh1-associated complex / nuclear pore localization / regulation of TORC1 signaling / nuclear pore outer ring / structural constituent of nuclear pore / vacuolar membrane / nucleocytoplasmic transport / ribosomal large subunit export from nucleus / mRNA transport / mRNA export from nucleus ...Seh1-associated complex / nuclear pore localization / regulation of TORC1 signaling / nuclear pore outer ring / structural constituent of nuclear pore / vacuolar membrane / nucleocytoplasmic transport / ribosomal large subunit export from nucleus / mRNA transport / mRNA export from nucleus / nuclear pore / positive regulation of TORC1 signaling / cellular response to amino acid starvation / protein import into nucleus / protein transport / nuclear envelope / nuclear membrane / positive regulation of DNA-templated transcription Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 3.5 Å | ||||||
Authors | Brohawn, S.G. / Leksa, N.C. / Rajashankar, K.R. / Schwartz, T.U. | ||||||
Citation | Journal: Science / Year: 2008 Title: Structural evidence for common ancestry of the nuclear pore complex and vesicle coats. Authors: Brohawn, S.G. / Leksa, N.C. / Spear, E.D. / Rajashankar, K.R. / Schwartz, T.U. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3ewe.cif.gz | 513.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3ewe.ent.gz | 423.7 KB | Display | PDB format |
PDBx/mmJSON format | 3ewe.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ew/3ewe ftp://data.pdbj.org/pub/pdb/validation_reports/ew/3ewe | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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3 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS ensembles :
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Details | HETEROTETRAMER IS NOT OBSERVED IN SOLUTION BY AUTHOR. SEE CITATION FOR DETAILS. |
-Components
#1: Protein | Mass: 39170.758 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: SEH1, YGL100W / Plasmid: pCola-Duet / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)-RIL / References: UniProt: P53011 #2: Protein | Mass: 64208.402 Da / Num. of mol.: 2 / Fragment: UNP residues 1-564 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: J1624, NUP85, RAT9, YJR042W / Plasmid: pCola-Duet / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)-RIL / References: UniProt: P46673 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.69 Å3/Da / Density % sol: 54.19 % |
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Crystal grow | Temperature: 289 K / pH: 8.5 Details: 18% PEG 3350, 0.1M Bis Tris propane, 0.2M Sodium Citrate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9792 |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Apr 22, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 3.4→40 Å / Num. obs: 29579 / % possible obs: 96.9 % / Observed criterion σ(I): 0 / Redundancy: 3.1 % / Biso Wilson estimate: 118 Å2 / Rsym value: 0.107 / Net I/σ(I): 13.7 |
Reflection shell | Resolution: 3.4→3.52 Å / Redundancy: 3.3 % / % possible all: 98.8 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 3.5→29.75 Å / SU ML: 0.59 / σ(F): 1.34 / Phase error: 38.69 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 102.79 Å2 / ksol: 0.26 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.58 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.5→29.75 Å
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Refine LS restraints |
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Refine LS restraints NCS |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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