+Open data
-Basic information
Entry | Database: PDB / ID: 3ejj | |||||||||
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Title | Structure of M-CSF bound to the first three domains of FMS | |||||||||
Components |
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Keywords | CYTOKINE/SIGNALING PROTEIN / growth factor-receptor complex / receptor tyrosine kinase / cytokine / 4-helix bundle / ATP-binding / Glycoprotein / Immunoglobulin domain / Kinase / Membrane / Nucleotide-binding / Phosphoprotein / Proto-oncogene / Receptor / Transferase / Transmembrane / Tyrosine-protein kinase / CYTOKINE-SIGNALING PROTEIN COMPLEX | |||||||||
Function / homology | Function and homology information positive regulation of protein metabolic process => GO:0051247 / regulation of mononuclear cell proliferation / negative regulation of platelet formation / Other interleukin signaling / mammary gland fat development / positive regulation of macrophage colony-stimulating factor signaling pathway / macrophage homeostasis / positive regulation of astrocyte activation / macrophage colony-stimulating factor receptor binding / monocyte homeostasis ...positive regulation of protein metabolic process => GO:0051247 / regulation of mononuclear cell proliferation / negative regulation of platelet formation / Other interleukin signaling / mammary gland fat development / positive regulation of macrophage colony-stimulating factor signaling pathway / macrophage homeostasis / positive regulation of astrocyte activation / macrophage colony-stimulating factor receptor binding / monocyte homeostasis / osteoclast proliferation / : / positive regulation of macrophage migration / developmental process involved in reproduction / macrophage colony-stimulating factor receptor activity / positive regulation of odontogenesis of dentin-containing tooth / forebrain neuron differentiation / CSF1-CSF1R complex / macrophage colony-stimulating factor signaling pathway / cell-cell junction maintenance / positive regulation of microglial cell migration / regulation of macrophage migration / mammary duct terminal end bud growth / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / Post-translational protein phosphorylation / positive regulation of lymphocyte proliferation / cellular response to macrophage colony-stimulating factor stimulus / negative regulation of myotube differentiation / positive regulation of cell cycle G1/S phase transition / organic cyclic compound binding / positive regulation of myoblast proliferation / microglial cell proliferation / positive regulation of macrophage differentiation / positive regulation of macrophage derived foam cell differentiation / olfactory bulb development / positive regulation of mononuclear cell proliferation / myeloid leukocyte migration / dentate gyrus development / positive regulation of mononuclear cell migration / positive regulation of leukocyte proliferation / positive regulation by host of viral process / neutrophil homeostasis / ruffle organization / positive regulation of multicellular organism growth / positive regulation of monocyte differentiation / positive regulation of osteoclast differentiation / microglia development / positive regulation of Ras protein signal transduction / positive regulation of macrophage proliferation / positive regulation of cell motility / regulation of bone resorption / branching involved in mammary gland duct morphogenesis / odontogenesis / positive regulation of leukocyte migration / positive regulation of cell-matrix adhesion / neuron projection extension / negative regulation of epithelial cell differentiation / positive regulation of macrophage chemotaxis / cytokine binding / growth factor binding / cellular response to cytokine stimulus / regulation of MAPK cascade / monocyte differentiation / hemopoiesis / macrophage differentiation / regulation of ossification / positive regulation of protein kinase activity / positive regulation of bone mineralization / homeostasis of number of cells within a tissue / positive regulation of chemokine production / positive regulation of tyrosine phosphorylation of STAT protein / positive regulation of protein metabolic process / transmembrane receptor protein tyrosine kinase activity / ossification / osteoclast differentiation / cytokine activity / response to ischemia / axon guidance / regulation of actin cytoskeleton organization / microglial cell activation / growth factor activity / receptor protein-tyrosine kinase / cytokine-mediated signaling pathway / peptidyl-tyrosine phosphorylation / cell surface receptor protein tyrosine kinase signaling pathway / positive regulation of tumor necrosis factor production / cell body / regulation of cell shape / perikaryon / protein phosphatase binding / protein tyrosine kinase activity / Ras protein signal transduction / cell population proliferation / protein autophosphorylation / membrane => GO:0016020 / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of ERK1 and ERK2 cascade / receptor complex / nuclear body / positive regulation of cell migration Similarity search - Function | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | |||||||||
Authors | Chen, X. / Liu, H. / Focia, P.J. / Shim, A. / He, X. | |||||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2008 Title: Structure of macrophage colony stimulating factor bound to FMS: diverse signaling assemblies of class III receptor tyrosine kinases. Authors: Chen, X. / Liu, H. / Focia, P.J. / Shim, A.H. / He, X. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3ejj.cif.gz | 145.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3ejj.ent.gz | 112.4 KB | Display | PDB format |
PDBx/mmJSON format | 3ejj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ej/3ejj ftp://data.pdbj.org/pub/pdb/validation_reports/ej/3ejj | HTTPS FTP |
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-Related structure data
Related structure data | 1hmcS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 18194.328 Da / Num. of mol.: 2 / Fragment: M-CSF (UNP residues 36 to 180) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Csf1 / Plasmid: pAcGP67A / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q3U395, UniProt: P07141*PLUS #2: Protein | | Mass: 32052.201 Da / Num. of mol.: 1 / Fragment: c-FMS (UNP residues 20 to 298) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Csf1r, Csfmr, Fms / Plasmid: pAcGP67A / Production host: Spodoptera frugiperda (fall armyworm) References: UniProt: P09581, receptor protein-tyrosine kinase #3: Polysaccharide | Source method: isolated from a genetically manipulated source #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.23 Å3/Da / Density % sol: 70.94 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 7.3 Details: 12% PEG 3000, 0.1M Hepes, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 21-ID-D / Wavelength: 0.9766 Å |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Jan 5, 2008 |
Radiation | Monochromator: na / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9766 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→20 Å / Num. all: 44786 / Num. obs: 44786 / % possible obs: 98.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.2 % / Biso Wilson estimate: 55.7 Å2 / Rmerge(I) obs: 0.042 / Net I/σ(I): 17.9 |
Reflection shell | Resolution: 2.4→2.55 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.467 / Mean I/σ(I) obs: 2.5 / Num. unique all: 6957 / % possible all: 98.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1HMC Resolution: 2.4→20 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 73 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.4→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.4→2.55 Å / Rfactor Rfree error: 0.023
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