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- PDB-3eau: Voltage-dependent K+ channel beta subunit in complex with cortisone -
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Open data
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Basic information
Entry | Database: PDB / ID: 3eau | ||||||
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Title | Voltage-dependent K+ channel beta subunit in complex with cortisone | ||||||
![]() | Voltage-gated potassium channel subunit beta-2![]() | ||||||
![]() | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Function / homology | ![]() pinceau fiber / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Pan, Y. / Weng, J. / Kabaleeswaran, V. / Li, H. / Cao, Y. / Bhosle, R.C. / Zhou, M. | ||||||
![]() | ![]() Title: Cortisone dissociates the Shaker family K+ channels from their beta subunits. Authors: Pan, Y. / Weng, J. / Kabaleeswaran, V. / Li, H. / Cao, Y. / Bhosle, R.C. / Zhou, M. #1: ![]() Title: Structure of a voltage-dependent K+ channel beta subunit. Authors: Gulbis, J.M. / Mann, S. / MacKinnon, R. #2: ![]() Title: Structure of the cytoplasmic beta subunit-T1 assembly of voltage-dependent K+ channels. Authors: Gulbis, J.M. / Zhou, M. / Mann, S. / MacKinnon, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 85.4 KB | Display | ![]() |
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PDB format | ![]() | 63 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3eb3C ![]() 3eb4C ![]() 1exbS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 36572.191 Da / Num. of mol.: 1 / Fragment: cytoplasmic Kvbeta subunit Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() | ||
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#2: Chemical | ChemComp-NDP / ![]() | ||
#3: Chemical | ![]() #4: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.21 Å3/Da / Density % sol: 61.68 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 6-15% glycerol, 1.5 M ammonium sulfate, 0.1 M Tris, VAPOR DIFFUSION, SITTING DROP, temperature 293K PH range: 7.9 - 8.8 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 16, 2007 / Details: mirrors |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.82→50 Å / Num. all: 81011 / Num. obs: 42550 / % possible obs: 98 % / Observed criterion σ(F): 1.5 / Observed criterion σ(I): 2 / Redundancy: 13.3 % / Biso Wilson estimate: 19.3 Å2 / Rmerge(I) obs: 0.061 / Rsym value: 0.051 / Net I/σ(I): 39 |
Reflection shell | Resolution: 1.82→1.92 Å / Redundancy: 10.4 % / Rmerge(I) obs: 0.396 / Mean I/σ(I) obs: 6.17 / Num. unique all: 3784 / Rsym value: 0.346 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: pdb entry 1EXB, chain A, beta subunit Resolution: 1.82→29.87 Å / Rfactor Rfree error: 0.004 / Data cutoff high absF: 3065704.25 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 2 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 39.5314 Å2 / ksol: 0.367486 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 26.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.82→29.87 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.82→1.92 Å / Rfactor Rfree error: 0.013 / Total num. of bins used: 6
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Xplor file |
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