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Yorodumi- PDB-3dps: X-ray structure of the unliganded uridine phosphorylase from salm... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3dps | ||||||
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Title | X-ray structure of the unliganded uridine phosphorylase from salmonella typhimurium in homodimeric form at 1.8A | ||||||
Components | Uridine phosphorylase | ||||||
Keywords | TRANSFERASE / Glycosyltransferase | ||||||
Function / homology | Function and homology information nucleoside catabolic process / uridine phosphorylase / nucleotide catabolic process / UMP salvage / uridine phosphorylase activity / cytosol Similarity search - Function | ||||||
Biological species | Salmonella typhimurium (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.8 Å | ||||||
Authors | Mikhailov, A.M. / Lashkov, A.A. | ||||||
Citation | Journal: To be Published Title: X-ray structure of the unliganded uridine phosphorylase from salmonella typhimurium in homodimeric form at 1.8A Authors: Lashkov, A.A. / Mikhailov, A.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3dps.cif.gz | 107.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3dps.ent.gz | 81.6 KB | Display | PDB format |
PDBx/mmJSON format | 3dps.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dp/3dps ftp://data.pdbj.org/pub/pdb/validation_reports/dp/3dps | HTTPS FTP |
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-Related structure data
Related structure data | 2oxfS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The biological assembly is a hexamer generated from the dimer in the asymmetric unit by the operations: 1-y, x-y, z and 1-x+y, x-1, z. |
-Components
#1: Protein | Mass: 27169.092 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Salmonella typhimurium (bacteria) / Strain: LT2 / Gene: udp / Plasmid: PBLUESCRIPT IISK / Production host: Escherichia coli (E. coli) / Strain (production host): Bl21 De3 / References: UniProt: P0A1F6, uridine phosphorylase #2: Chemical | ChemComp-GOL / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.93 Å3/Da / Density % sol: 36.21 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 5.2 Details: PEG 400, NaN3, GLYCEROL, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: X13 / Wavelength: 0.813 Å |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Nov 23, 2007 |
Radiation | Monochromator: GAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.813 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→20 Å / Num. all: 37630 / Num. obs: 37497 / % possible obs: 99.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Biso Wilson estimate: 19.14 Å2 / Rmerge(I) obs: 0.058 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2OXF Resolution: 1.8→18.696 Å / Occupancy max: 1 / Occupancy min: 0.5 / SU ML: 0.21 / σ(F): 2.01 / Phase error: 18.18 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 51.232 Å2 / ksol: 0.378 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 162.77 Å2 / Biso mean: 25.463 Å2 / Biso min: 7.69 Å2
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Refinement step | Cycle: LAST / Resolution: 1.8→18.696 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 13
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