+Open data
-Basic information
Entry | Database: PDB / ID: 3ctb | ||||||
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Title | Tethered PXR-LBD/SRC-1p apoprotein | ||||||
Components | Pregnane X receptor, Linker, Steroid receptor coactivator 1 | ||||||
Keywords | Transcription / Transferase / pxr / src-1 / tethered / engineered / drug-drug interactions / DNA-binding / Metal-binding / Nucleus / Receptor / Transcription regulation / Zinc-finger | ||||||
Function / homology | Function and homology information labyrinthine layer morphogenesis / xenobiotic transport / regulation of thyroid hormone mediated signaling pathway / positive regulation of transcription from RNA polymerase II promoter by galactose / positive regulation of female receptivity / intermediate filament cytoskeleton / hypothalamus development / male mating behavior / NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis / steroid metabolic process ...labyrinthine layer morphogenesis / xenobiotic transport / regulation of thyroid hormone mediated signaling pathway / positive regulation of transcription from RNA polymerase II promoter by galactose / positive regulation of female receptivity / intermediate filament cytoskeleton / hypothalamus development / male mating behavior / NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis / steroid metabolic process / estrous cycle / cellular response to Thyroglobulin triiodothyronine / Synthesis of bile acids and bile salts / Endogenous sterols / Synthesis of bile acids and bile salts via 27-hydroxycholesterol / Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol / xenobiotic catabolic process / nuclear retinoid X receptor binding / response to retinoic acid / histone acetyltransferase activity / regulation of cellular response to insulin stimulus / Recycling of bile acids and salts / histone acetyltransferase / cellular response to hormone stimulus / NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux / positive regulation of adipose tissue development / peroxisome proliferator activated receptor signaling pathway / RORA activates gene expression / lactation / positive regulation of neuron differentiation / Regulation of lipid metabolism by PPARalpha / xenobiotic metabolic process / cerebellum development / BMAL1:CLOCK,NPAS2 activates circadian gene expression / SUMOylation of transcription cofactors / Activation of gene expression by SREBF (SREBP) / nuclear receptor coactivator activity / response to progesterone / nuclear estrogen receptor binding / nuclear receptor binding / hippocampus development / RNA polymerase II transcription regulatory region sequence-specific DNA binding / Heme signaling / SUMOylation of intracellular receptors / mRNA transcription by RNA polymerase II / Transcriptional activation of mitochondrial biogenesis / PPARA activates gene expression / Cytoprotection by HMOX1 / cerebral cortex development / Transcriptional regulation of white adipocyte differentiation / Nuclear Receptor transcription pathway / RNA polymerase II transcription regulator complex / male gonad development / nuclear receptor activity / Circadian Clock / sequence-specific double-stranded DNA binding / response to estradiol / HATs acetylate histones / DNA-binding transcription activator activity, RNA polymerase II-specific / Estrogen-dependent gene expression / transcription regulator complex / cell differentiation / transcription coactivator activity / nuclear body / protein dimerization activity / DNA-binding transcription factor activity, RNA polymerase II-specific / positive regulation of apoptotic process / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of DNA-templated transcription / chromatin binding / chromatin / positive regulation of gene expression / regulation of DNA-templated transcription / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / signal transduction / positive regulation of transcription by RNA polymerase II / protein-containing complex / zinc ion binding / nucleoplasm / nucleus / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Lesburg, C.A. | ||||||
Citation | Journal: Protein Eng.Des.Sel. / Year: 2008 Title: Construction and characterization of a fully active PXR/SRC-1 tethered protein with increased stability Authors: Wang, W. / Prosise, W.W. / Chen, J. / Taremi, S.S. / Le, H.V. / Madison, V. / Cui, X. / Thomas, A. / Cheng, K.C. / Lesburg, C.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3ctb.cif.gz | 138.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3ctb.ent.gz | 107.2 KB | Display | PDB format |
PDBx/mmJSON format | 3ctb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ct/3ctb ftp://data.pdbj.org/pub/pdb/validation_reports/ct/3ctb | HTTPS FTP |
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-Related structure data
Related structure data | 3hvlC 1nrlS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 39184.035 Da / Num. of mol.: 2 Fragment: PXR, residues 130-434, linker, SRC-1, residues 678-700 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) Description: THE PEPTIDE FROM STEROID RECEPTOR COACTIVATOR 1 WAS CHEMICALLY SYNTHESIZED. THE SEQUENCE OF THE PEPTIDE IS NATURALLY FOUND IN HOMO SAPIENS (HUMAN). Gene: NR1I2, PXR / Production host: Escherichia coli (E. coli) References: UniProt: O75469, UniProt: Q15788, histone acetyltransferase #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.55 Å3/Da / Density % sol: 51.83 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion / pH: 7 Details: 10-30% MPD or isopropanol, 100 mM imidazole, pH 7, VAPOR DIFFUSION, temperature 277K |
-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU FR-E+ SUPERBRIGHT / Wavelength: 1.54 Å |
Detector | Type: RIGAKU RAXIS HTC / Detector: IMAGE PLATE / Date: May 26, 2005 / Details: VariMax HF |
Radiation | Monochromator: multilayer mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 2→100 Å / Num. obs: 54259 / % possible obs: 98.7 % / Observed criterion σ(F): 3 / Observed criterion σ(I): 3 / Redundancy: 3.58 % / Biso Wilson estimate: 44.07 Å2 / Rmerge(I) obs: 0.039 / Rsym value: 0.039 / Net I/σ(I): 13.2 |
Reflection shell | Resolution: 2→2.07 Å / Redundancy: 3.49 % / Rmerge(I) obs: 0.461 / Mean I/σ(I) obs: 2.1 / Num. unique all: 5082 / Rsym value: 0.461 / % possible all: 96.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 1nrl Resolution: 2→45.31 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 52.08 Å2
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Refine analyze | Luzzati coordinate error obs: 0.2555 Å | |||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→45.31 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.12 Å / Total num. of bins used: 9
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