Resolution: 2.3→40.26 Å / Cor.coef. Fo:Fc: 0.95 / Cor.coef. Fo:Fc free: 0.928 / SU B: 10.808 / SU ML: 0.144 / Cross valid method: THROUGHOUT / ESU R: 0.231 / ESU R Free: 0.192 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. RESIDUES 250-277 (_C TAIL) WERE NOT VISIBLE IN THE ELECTRON DENSITY. HOWEVER, SDS-PAGE ON A DISSOLVED CRYSTAL INDICATES THAT THE PROTEIN ...Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. RESIDUES 250-277 (_C TAIL) WERE NOT VISIBLE IN THE ELECTRON DENSITY. HOWEVER, SDS-PAGE ON A DISSOLVED CRYSTAL INDICATES THAT THE PROTEIN WAS INTACT, AND THEREFORE WE ASSUME THE MISSING RESIDUES ARE HIGHLY DISORDERED OR MOBILE AND THUS NOT VISIBLE IN ELECTRON DENSITY. THERE IS A 0.25 OCCUPANCY COBALT ION MODELLED ADJACENT TO HIS203. THE CRYSTAL USED FOR REFINEMENT WAS SOAK IN A MOTHER LIQUOR SUPPLEMENTED WITH 10MM COBALT TO TRY AND LOCATE POSSIBLE COBALT BINDING SITES. WHILST THIS SITE IS VISIBLE (CONFIRMED WITH AN ANOMALOUS DIFFERENCE FOURIER), WE DO NOT BELIEVE THAT THIS SITE IS BIOLOGICALLY RELEVANT. SEE PUBLICATION FOR DETAILS.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.228
395
4.6 %
RANDOM
Rwork
0.191
-
-
-
obs
0.193
8221
98.4 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK