SIGNALING PROTEIN / PHOSPHOLIPASE C / PHOSPHOINOSITIDES / RHO GTPASES / RAC SIGNALING PROTEIN
Function / homology
Function and homology information
regulation of mast cell chemotaxis / regulation of cell-substrate adhesion / lymphocyte aggregation / erythrocyte enucleation / positive regulation of mast cell proliferation / regulation of respiratory burst / regulation of neutrophil migration / mast cell proliferation / regulation of mast cell degranulation / regulation of hydrogen peroxide metabolic process ...regulation of mast cell chemotaxis / regulation of cell-substrate adhesion / lymphocyte aggregation / erythrocyte enucleation / positive regulation of mast cell proliferation / regulation of respiratory burst / regulation of neutrophil migration / mast cell proliferation / regulation of mast cell degranulation / regulation of hydrogen peroxide metabolic process / NADPH oxidase complex / respiratory burst / ROS and RNS production in phagocytes / cell projection assembly / protein kinase regulator activity / PCP/CE pathway / positive regulation of neutrophil chemotaxis / positive regulation of protein targeting to mitochondrion / small GTPase-mediated signal transduction / regulation of T cell proliferation / RHO GTPases Activate NADPH Oxidases / RAC2 GTPase cycle / positive regulation of lamellipodium assembly / bone resorption / regulation of cell migration / GPVI-mediated activation cascade / actin filament organization / phagocytic vesicle membrane / Constitutive Signaling by Aberrant PI3K in Cancer / chemotaxis / PIP3 activates AKT signaling / lamellipodium / nuclear envelope / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / mitochondrial outer membrane / G protein-coupled receptor signaling pathway / focal adhesion / GTPase activity / endoplasmic reticulum membrane / GTP binding / signal transduction / extracellular exosome / plasma membrane / cytosol Similarity search - Function
Small GTPase Rho / small GTPase Rho family profile. / Ran (Ras-related nuclear proteins) /TC4 subfamily of small GTPases / Rho (Ras homology) subfamily of Ras-like small GTPases / Ras subfamily of RAS small GTPases / Small GTPase / Ras family / Rab subfamily of small GTPases / Small GTP-binding protein domain / P-loop containing nucleotide triphosphate hydrolases ...Small GTPase Rho / small GTPase Rho family profile. / Ran (Ras-related nuclear proteins) /TC4 subfamily of small GTPases / Rho (Ras homology) subfamily of Ras-like small GTPases / Ras subfamily of RAS small GTPases / Small GTPase / Ras family / Rab subfamily of small GTPases / Small GTP-binding protein domain / P-loop containing nucleotide triphosphate hydrolases / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 137 / Source method: isolated from a natural source / Formula: H2O
Compound details
ENGINEERED RESIDUE IN CHAIN A, GLY 12 TO VAL
Sequence details
THERE IS A LEADING LINKER SEQUENCE GGGSGGS (NOT SEEN IN STRUCTURE) AND MUTATION G12V
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.55 Å3/Da / Density % sol: 52 % / Description: NONE
Crystal grow
Temperature: 277 K / pH: 7 Details: RAC2(2-192)GDP WAS CRYSTALLIZED USING A PROTEIN CONCENTRATION OF 20 MG/ML WITH PRECIPITANT (20% PEG3350, 100 MM BIS-TRIS PROPANE PH 7.0) AND AT A CONSTANT TEMPERATURE OF 4C
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.9796 Å / Relative weight: 1
Reflection
Resolution: 1.95→54 Å / Num. obs: 79004 / % possible obs: 98.7 % / Redundancy: 4.8 % / Rmerge(I) obs: 0.08 / Net I/σ(I): 12.9
Reflection shell
Resolution: 1.95→2.06 Å / Redundancy: 4.5 % / Rmerge(I) obs: 0.52 / Mean I/σ(I) obs: 2.3 / % possible all: 96.1
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Processing
Software
Name
Version
Classification
PHENIX
(PHENIX.REFINE)
refinement
MOSFLM
datareduction
SCALA
datascaling
PHASER
phasing
Refinement
Method to determine structure: MOLECULAR REPLACEMENT Starting model: IN HOUSE STRUCTURE. Resolution: 1.95→54.03 Å / SU ML: 0.31 / σ(F): 1.21 / Phase error: 30.75 / Stereochemistry target values: ML
Rfactor
Num. reflection
% reflection
Rfree
0.254
1535
5 %
Rwork
0.207
-
-
obs
0.21
30557
96.7 %
Solvent computation
Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 59.89 Å2 / ksol: 0.36 e/Å3
Displacement parameters
Baniso -1
Baniso -2
Baniso -3
1-
-12.854 Å2
0 Å2
-0 Å2
2-
-
27.7227 Å2
-0 Å2
3-
-
-
-18.1119 Å2
Refinement step
Cycle: LAST / Resolution: 1.95→54.03 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
1336
0
29
137
1502
Refine LS restraints
Refine-ID
Type
Dev ideal
Number
X-RAY DIFFRACTION
f_bond_d
0.023
1407
X-RAY DIFFRACTION
f_angle_d
2.048
1936
X-RAY DIFFRACTION
f_dihedral_angle_d
19.078
490
X-RAY DIFFRACTION
f_chiral_restr
0.134
231
X-RAY DIFFRACTION
f_plane_restr
0.012
242
LS refinement shell
Resolution (Å)
Rfactor Rfree
Num. reflection Rfree
Rfactor Rwork
Num. reflection Rwork
Refine-ID
% reflection obs (%)
1.9501-2.013
0.3542
142
0.3598
2446
X-RAY DIFFRACTION
90
2.013-2.085
0.3172
139
0.3028
2533
X-RAY DIFFRACTION
93
2.085-2.1685
0.2956
141
0.2813
2563
X-RAY DIFFRACTION
94
2.1685-2.2672
0.3357
141
0.262
2585
X-RAY DIFFRACTION
95
2.2672-2.3867
0.3502
150
0.2355
2621
X-RAY DIFFRACTION
97
2.3867-2.5362
0.2563
131
0.216
2728
X-RAY DIFFRACTION
98
2.5362-2.7321
0.2881
123
0.2116
2720
X-RAY DIFFRACTION
100
2.7321-3.007
0.2457
135
0.2047
2721
X-RAY DIFFRACTION
99
3.007-3.442
0.2257
163
0.1924
2708
X-RAY DIFFRACTION
99
3.442-4.3363
0.2376
160
0.1678
2683
X-RAY DIFFRACTION
99
4.3363-54.0528
0.1742
110
0.1646
2714
X-RAY DIFFRACTION
98
+
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