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Yorodumi- PDB-2v9h: Solution Structure of an Escherichia coli YaeT tandem POTRA domain -
+Open data
-Basic information
Entry | Database: PDB / ID: 2v9h | ||||||
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Title | Solution Structure of an Escherichia coli YaeT tandem POTRA domain | ||||||
Components | OUTER MEMBRANE PROTEIN ASSEMBLY FACTOR YAET | ||||||
Keywords | PROTEIN BINDING / YAET / MEMBRANE / POTRA DOMAIN / OUTER MEMBRANE / OUTER MEMBRANE PROTEIN FOLDING / PROTEIN-BINDING | ||||||
Function / homology | Function and homology information Bam protein complex / Gram-negative-bacterium-type cell outer membrane assembly / protein insertion into membrane / cell outer membrane / cell adhesion / membrane Similarity search - Function | ||||||
Biological species | ESCHERICHIA COLI (E. coli) | ||||||
Method | SOLUTION NMR / distance geometry | ||||||
Authors | Knowles, T.J. / Jeeves, M. / Bobat, S. / Dancea, F. / Mcclelland, D.M. / Palmer, T. / Overduin, M. / Henderson, I.R. | ||||||
Citation | Journal: Mol.Microbiol. / Year: 2008 Title: Fold and Function of Polypeptide Transport-Associated Domains Responsible for Delivering Unfolded Proteins to Membranes. Authors: Knowles, T.J. / Jeeves, M. / Bobat, S. / Dancea, F. / Mcclelland, D.M. / Palmer, T. / Overduin, M. / Henderson, I.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2v9h.cif.gz | 926.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2v9h.ent.gz | 781.8 KB | Display | PDB format |
PDBx/mmJSON format | 2v9h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/v9/2v9h ftp://data.pdbj.org/pub/pdb/validation_reports/v9/2v9h | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 17991.270 Da / Num. of mol.: 1 / Fragment: POTRA DOMAINS 1 AND 2, RESIDUES 21-174 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ESCHERICHIA COLI (E. coli) / Strain: MC4100 / Plasmid: PQE70 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): M15PREP4 / References: UniProt: P0A940 |
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Sequence details | RESIDUES 21-174 PRESENTED HERE |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELED YAET21-174 |
-Sample preparation
Details | Contents: 50 MM MES, 50 MM NACL |
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Sample conditions | Ionic strength: 86 mM / pH: 6.5 / Pressure: 1.0 atm / Temperature: 303.0 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: distance geometry / Software ordinal: 1 | |||||||||||||||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATIONS / Conformers calculated total number: 450 / Conformers submitted total number: 20 |