[English] 日本語
![](img/lk-miru.gif)
- PDB-2v36: Crystal structure of gamma-glutamyl transferase from Bacillus subtilis -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 2v36 | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of gamma-glutamyl transferase from Bacillus subtilis | ||||||
![]() |
| ||||||
![]() | ![]() ![]() ![]() | ||||||
Function / homology | ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sharath, B. / Prabhune, A.A. / Suresh, C.G. / Wilkinson, A.J. / Brannigan, J.A. | ||||||
![]() | ![]() Title: Crystal Structure of Gamma-Glutamyl Transferase Authors: Sharath, B. / Prabhune, A.A. / Suresh, C.G. / Wilkinson, A.J. / Brannigan, J.A. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 216.1 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 170.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 2dbuS S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 41452.953 Da / Num. of mol.: 2 / Fragment: RESIDUES 29-402 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 21100.697 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Water | ChemComp-HOH / | ![]() |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 54.2 % / Description: NONE |
---|---|
Crystal grow![]() | pH: 7.5 Details: 0.1M SODIUM HEPES PH 7.5, 25% PEG 2000K MME AND 0.2M KSCN |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.84→29.6 Å / Num. obs: 1952022 / % possible obs: 97.8 % / Observed criterion σ(I): 4.5 / Redundancy: 4.4 % / Rmerge(I) obs: 0.1 / Net I/σ(I): 13.8 |
Reflection shell | Resolution: 1.85→1.92 Å / Redundancy: 2.6 % / Rmerge(I) obs: 0.44 / Mean I/σ(I) obs: 1.9 / % possible all: 82.7 |
-
Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 2DBU Resolution: 1.85→90.17 Å / Cor.coef. Fo:Fc: 0.923 / Cor.coef. Fo:Fc free: 0.905 / SU B: 2.916 / SU ML: 0.091 / Cross valid method: THROUGHOUT / ESU R: 0.119 / ESU R Free: 0.135 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. DISORDERED REGIONS NOT MODELED
| ||||||||||||||||||||
Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||
Displacement parameters | Biso mean: 17.48 Å2
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.85→90.17 Å
| ||||||||||||||||||||
LS refinement shell | Resolution: 1.84→1.89 Å / Total num. of bins used: 20 /
|