+Open data
-Basic information
Entry | Database: PDB / ID: 2pxg | ||||||
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Title | NMR Solution Structure of OmlA | ||||||
Components | Outer membrane proteinVirulence-related outer membrane protein family | ||||||
Keywords | MEMBRANE PROTEIN / two layer alpha/beta plait / two layer sandwich architecture | ||||||
Function / homology | Function and homology information Gram-negative-bacterium-type cell outer membrane assembly / protein insertion into membrane / cell outer membrane Similarity search - Function | ||||||
Biological species | Xanthomonas axonopodis pv. citri (bacteria) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Vanini, M.M.T. / Pertinhez, T.A. / Sforca, M.L. / Spisni, A. / Benedetti, C.E. | ||||||
Citation | Journal: Proteins / Year: 2008 Title: The solution structure of the outer membrane lipoprotein OmlA from Xanthomonas axonopodis pv. citri reveals a protein fold implicated in protein-protein interaction. Authors: Vanini, M.M. / Spisni, A. / Sforca, M.L. / Pertinhez, T.A. / Benedetti, C.E. #1: Journal: J.Biomol.NMR / Year: 2006 Title: NMR Assignment of the Outer Membrane Lipoprotein (OmlA) from Xanthomonas axonopodis pv citri Authors: Vanini, M.M.T. / Benedetti, C.E. / Spisni, A. / Pertinhez, T.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2pxg.cif.gz | 752.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2pxg.ent.gz | 628.1 KB | Display | PDB format |
PDBx/mmJSON format | 2pxg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/px/2pxg ftp://data.pdbj.org/pub/pdb/validation_reports/px/2pxg | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13602.290 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Xanthomonas axonopodis pv. citri (bacteria) Species: Xanthomonas axonopodisCitrus canker / Strain: 306 isolate / Gene: smpA / Plasmid: pET28a / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q8PMB6 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | pH: 5.8 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 Details: the structures are based on a total of 1115 NOE-derived distance constraints | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 20 / Conformers submitted total number: 20 |