microlipophagy / positive regulation of protein maturation / ESCRT-0 complex / microautophagy / ATP export / protein retention in Golgi apparatus / protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway / multivesicular body sorting pathway / protein targeting to vacuole / late endosome to vacuole transport ...microlipophagy / positive regulation of protein maturation / ESCRT-0 complex / microautophagy / ATP export / protein retention in Golgi apparatus / protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway / multivesicular body sorting pathway / protein targeting to vacuole / late endosome to vacuole transport / ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway / phosphatidylinositol-3-phosphate binding / vacuolar membrane / protein secretion / ubiquitin binding / endosome membrane / エンドソーム / protein heterodimerization activity / protein domain specific binding / protein-containing complex / metal ion binding 類似検索 - 分子機能
Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #1940 / : / Vacuolar protein sorting-associated protein 27, GAT-like domain / Hepatocyte growth factor-regulated tyrosine kinase substrate/VPS27 / FYVE zinc finger / FYVE zinc finger / Protein present in Fab1, YOTB, Vac1, and EEA1 / VHS domain / VHS domain / VHS domain profile. ...Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #1940 / : / Vacuolar protein sorting-associated protein 27, GAT-like domain / Hepatocyte growth factor-regulated tyrosine kinase substrate/VPS27 / FYVE zinc finger / FYVE zinc finger / Protein present in Fab1, YOTB, Vac1, and EEA1 / VHS domain / VHS domain / VHS domain profile. / Domain present in VPS-27, Hrs and STAM / Zinc finger, FYVE-related / Zinc finger FYVE/FYVE-related type profile. / Ubiquitin interaction motif / ENTH/VHS / Ubiquitin-interacting motif. / Ubiquitin interacting motif / Ubiquitin-interacting motif (UIM) domain profile. / SH3ドメイン / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / Zinc finger, FYVE/PHD-type / Src homology 3 domains / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3ドメイン / Zinc finger, RING/FYVE/PHD-type / Up-down Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
Class E vacuolar protein-sorting machinery protein HSE1 / Vacuolar protein sorting-associated protein 27 類似検索 - 構成要素
温度: 288 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 8.85 詳細: 1.1-1.4 M ammonium sulfate, 0.1 M Tris-HCl. Then micro-seeding into 1.25 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.85, VAPOR DIFFUSION, SITTING DROP, temperature 288K
解像度: 3.01→46.92 Å / Num. obs: 7506 / 冗長度: 4.1 % / Rsym value: 0.052 / Net I/σ(I): 18.1
反射 シェル
解像度: 3.01→3.14 Å
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解析
ソフトウェア
名称
バージョン
分類
CNS
1.1
精密化
HKL-2000
データ収集
DENZO
データ削減
HKL-2000
データスケーリング
SnB
位相決定
精密化
構造決定の手法: TWO WAVELENGTH MAD / 解像度: 3.01→46.92 Å / Rfactor Rfree error: 0.015 / Data cutoff high absF: 276902.72 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / 交差検証法: THROUGHOUT / σ(F): 0 / 立体化学のターゲット値: Engh & Huber 詳細: BULK SOLVENT MODEL USED. The Friedel pairs were used for phasing.