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Yorodumi- PDB-2n4i: The solution structure of Skint-1, a critical determinant of dend... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2n4i | ||||||
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Title | The solution structure of Skint-1, a critical determinant of dendritic epidermal gamma-delta T cell selection | ||||||
Components | Selection and upkeep of intraepithelial T-cells protein 1 | ||||||
Keywords | SIGNALING PROTEIN / Immune stress surveillance / thymic organ culture | ||||||
Function / homology | Function and homology information positive regulation of T cell differentiation in thymus / positive thymic T cell selection / positive regulation of T cell differentiation / regulation of immune response / regulation of cytokine production / cell periphery / T cell receptor signaling pathway / membrane => GO:0016020 / external side of plasma membrane / signaling receptor binding / membrane Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / simulated annealing, torsion angle dynamics | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Salim, M. / Knowles, T.J. / Hart, R. / Mohammed, F. / Woodward, M.J. / Willcox, C.R. / Overduin, M. / Hayday, A.C. / Willcox, B.E. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2016 Title: Characterization of a Putative Receptor Binding Surface on Skint-1, a Critical Determinant of Dendritic Epidermal T Cell Selection. Authors: Salim, M. / Knowles, T.J. / Hart, R. / Mohammed, F. / Woodward, M.J. / Willcox, C.R. / Overduin, M. / Hayday, A.C. / Willcox, B.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2n4i.cif.gz | 724.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2n4i.ent.gz | 629 KB | Display | PDB format |
PDBx/mmJSON format | 2n4i.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n4/2n4i ftp://data.pdbj.org/pub/pdb/validation_reports/n4/2n4i | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13569.285 Da / Num. of mol.: 1 / Fragment: Ig-like V-type domain residues 24-141 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Skint1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: A7TZE6 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 19 mg/mL [U-99% 13C; U-99% 15N] protein, 20 mM MES, 50 mM sodium chloride, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 50 / pH: 6.5 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |