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Yorodumi- PDB-2n05: Solution Structure of the non-phosphorylated N-terminal region of... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2n05 | ||||||
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Title | Solution Structure of the non-phosphorylated N-terminal region of Human Cysteine String Protein (CSP) | ||||||
Components | DnaJ homolog subfamily C member 5 | ||||||
Keywords | CHAPERONE / phosphorylation / DnaJ | ||||||
Function / homology | Function and homology information clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane / chromaffin granule membrane / regulated exocytosis / GABA synthesis, release, reuptake and degradation / regulation of synaptic vesicle cycle / ATP-dependent protein binding / Sensory processing of sound by inner hair cells of the cochlea / azurophil granule membrane / exocytosis / synaptic vesicle exocytosis ...clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane / chromaffin granule membrane / regulated exocytosis / GABA synthesis, release, reuptake and degradation / regulation of synaptic vesicle cycle / ATP-dependent protein binding / Sensory processing of sound by inner hair cells of the cochlea / azurophil granule membrane / exocytosis / synaptic vesicle exocytosis / specific granule membrane / chaperone-mediated protein folding / neuromuscular junction / synaptic vesicle membrane / melanosome / presynapse / neuron apoptotic process / negative regulation of neuron apoptotic process / lysosomal membrane / intracellular membrane-bounded organelle / Neutrophil degranulation / Golgi apparatus / mitochondrion / membrane / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Patel, P. / Lian, L. / Morgan, A. / Burgoyne, R. | ||||||
Citation | Journal: Structure / Year: 2016 Title: Phosphorylation of Cysteine String Protein Triggers a Major Conformational Switch. Authors: Patel, P. / Prescott, G.R. / Burgoyne, R.D. / Lian, L.Y. / Morgan, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2n05.cif.gz | 709.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2n05.ent.gz | 606.5 KB | Display | PDB format |
PDBx/mmJSON format | 2n05.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n0/2n05 ftp://data.pdbj.org/pub/pdb/validation_reports/n0/2n05 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11904.221 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: DNAJC5, CSP / Production host: Escherichia coli (E. coli) / References: UniProt: Q9H3Z4 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.15 / pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |