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Yorodumi- PDB-2lsp: solution structures of BRD4 second bromodomain with NF-kB-K310ac ... -
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-Basic information
Entry | Database: PDB / ID: 2lsp | |||||||||
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Title | solution structures of BRD4 second bromodomain with NF-kB-K310ac peptide | |||||||||
Components |
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Keywords | PROTEIN BINDING / NF-kB / BRD4 / HIV / bromodomain / kidney disease | |||||||||
Function / homology | Function and homology information acetaldehyde metabolic process / prolactin signaling pathway / DEx/H-box helicases activate type I IFN and inflammatory cytokines production / NF-kappaB p50/p65 complex / IkBA variant leads to EDA-ID / positive regulation of Schwann cell differentiation / cellular response to peptidoglycan / Regulated proteolysis of p75NTR / ankyrin repeat binding / RIP-mediated NFkB activation via ZBP1 ...acetaldehyde metabolic process / prolactin signaling pathway / DEx/H-box helicases activate type I IFN and inflammatory cytokines production / NF-kappaB p50/p65 complex / IkBA variant leads to EDA-ID / positive regulation of Schwann cell differentiation / cellular response to peptidoglycan / Regulated proteolysis of p75NTR / ankyrin repeat binding / RIP-mediated NFkB activation via ZBP1 / SUMOylation of immune response proteins / CLEC7A/inflammasome pathway / negative regulation of protein sumoylation / postsynapse to nucleus signaling pathway / defense response to tumor cell / nucleotide-binding oligomerization domain containing 2 signaling pathway / Interleukin-1 processing / cellular response to interleukin-6 / actinin binding / negative regulation of non-canonical NF-kappaB signal transduction / NF-kappaB complex / cellular response to angiotensin / response to UV-B / vascular endothelial growth factor signaling pathway / interleukin-1-mediated signaling pathway / Regulation of NFE2L2 gene expression / positive regulation of leukocyte adhesion to vascular endothelial cell / positive regulation of miRNA metabolic process / toll-like receptor 4 signaling pathway / cellular response to hepatocyte growth factor stimulus / positive regulation of amyloid-beta formation / positive regulation of T cell receptor signaling pathway / response to cobalamin / phosphate ion binding / non-canonical NF-kappaB signal transduction / cellular response to lipoteichoic acid / TRAF6 mediated NF-kB activation / response to muramyl dipeptide / RNA polymerase II C-terminal domain binding / The NLRP3 inflammasome / negative regulation of DNA damage checkpoint / general transcription initiation factor binding / Transcriptional Regulation by VENTX / P-TEFb complex binding / NF-kappaB binding / hair follicle development / neuropeptide signaling pathway / positive regulation of vascular endothelial growth factor production / negative regulation by host of viral transcription / RNA polymerase II core promoter sequence-specific DNA binding / canonical NF-kappaB signal transduction / response to amino acid / positive regulation of T-helper 17 cell lineage commitment / cellular response to interleukin-1 / cellular defense response / Purinergic signaling in leishmaniasis infection / negative regulation of insulin receptor signaling pathway / response to cAMP / tumor necrosis factor-mediated signaling pathway / positive regulation of G2/M transition of mitotic cell cycle / response to muscle stretch / histone reader activity / positive regulation of interleukin-12 production / NF-kB is activated and signals survival / CD209 (DC-SIGN) signaling / response to interleukin-1 / negative regulation of angiogenesis / RNA polymerase II CTD heptapeptide repeat kinase activity / negative regulation of miRNA transcription / liver development / response to progesterone / condensed nuclear chromosome / response to organic substance / positive regulation of interleukin-1 beta production / response to cytokine / response to ischemia / positive regulation of interleukin-8 production / Dectin-1 mediated noncanonical NF-kB signaling / negative regulation of extrinsic apoptotic signaling pathway / positive regulation of transcription elongation by RNA polymerase II / transcription coregulator activity / Activation of NF-kappaB in B cells / peptide binding / RNA polymerase II transcription regulatory region sequence-specific DNA binding / animal organ morphogenesis / protein catabolic process / TAK1-dependent IKK and NF-kappa-B activation / lysine-acetylated histone binding / response to insulin / transcription coactivator binding / negative regulation of protein catabolic process / chromatin DNA binding / cellular response to hydrogen peroxide / PKMTs methylate histone lysines / CLEC7A (Dectin-1) signaling / DNA-binding transcription repressor activity, RNA polymerase II-specific / FCERI mediated NF-kB activation / positive regulation of miRNA transcription / Transcriptional regulation of white adipocyte differentiation / Interleukin-1 signaling Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) Homo Sapiens (human) | |||||||||
Method | SOLUTION NMR / DGSA-distance geometry simulated annealing, torsion angle dynamics | |||||||||
Authors | Zhang, G. / Liu, R. / Zhong, Y. / Plotnikov, A.N. / Zhang, W. / Rusinova, E. / Gerona-Nevarro, G. / Moshkina, N. / Joshua, J. / Chuang, P.Y. ...Zhang, G. / Liu, R. / Zhong, Y. / Plotnikov, A.N. / Zhang, W. / Rusinova, E. / Gerona-Nevarro, G. / Moshkina, N. / Joshua, J. / Chuang, P.Y. / Ohlmeyer, M. / He, J. / Zhou, M.-M. | |||||||||
Citation | Journal: J.Biol.Chem. / Year: 2012 Title: Down-regulation of NF-kappa B transcriptional activity in HIV-associated kidney disease by BRD4 inhibition. Authors: Zhang, G. / Liu, R. / Zhong, Y. / Plotnikov, A.N. / Zhang, W. / Zeng, L. / Rusinova, E. / Gerona-Nevarro, G. / Moshkina, N. / Joshua, J. / Chuang, P.Y. / Ohlmeyer, M. / He, J.C. / Zhou, M.M. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2lsp.cif.gz | 887.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2lsp.ent.gz | 740.3 KB | Display | PDB format |
PDBx/mmJSON format | 2lsp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ls/2lsp ftp://data.pdbj.org/pub/pdb/validation_reports/ls/2lsp | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1663.956 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo Sapiens (human) / References: UniProt: Q04206 |
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#2: Protein | Mass: 14842.058 Da / Num. of mol.: 1 / Fragment: second bromodomain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: BRD4, HUNK1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: O60885 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: DGSA-distance geometry simulated annealing, torsion angle dynamics Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |