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Yorodumi- PDB-2lf6: Solution NMR structure of HopABPph1448_220_320 from Pseudomonas s... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2lf6 | ||||||
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Title | Solution NMR structure of HopABPph1448_220_320 from Pseudomonas syringae pv. phaseolicola str. 1448A, Midwest Center for Structural Genomics target APC40132.4 and Northeast Structural Genomics Consortium target PsT3A | ||||||
Components | Effector protein hopAB1 | ||||||
Keywords | SIGNALING PROTEIN / type III effector / Structural Genomics / PSI-Biology / Protein Structure Initiative / Northeast Structural Genomics Consortium / NESG / Midwest Center for Structural Genomics / MCSG / Ontario Centre for Structural Proteomics / OCSP | ||||||
Function / homology | Function and homology information symbiont-mediated perturbation of host defense-related programmed cell death / : / extracellular region Similarity search - Function | ||||||
Biological species | Pseudomonas syringae pv. phaseolicola 1448A (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Wu, B. / Yee, A. / Houliston, S. / Semesi, A. / Garcia, M. / Singer, A.U. / Savchenko, A. / Montelione, G.T. / Joachimiak, A. / Arrowsmith, C.H. ...Wu, B. / Yee, A. / Houliston, S. / Semesi, A. / Garcia, M. / Singer, A.U. / Savchenko, A. / Montelione, G.T. / Joachimiak, A. / Arrowsmith, C.H. / Northeast Structural Genomics Consortium (NESG) / Midwest Center for Structural Genomics (MCSG) / Ontario Centre for Structural Proteomics (OCSP) | ||||||
Citation | Journal: Biochemistry / Year: 2012 Title: Structural Analysis of HopPmaL Reveals the Presence of a Second Adaptor Domain Common to the HopAB Family of Pseudomonas syringae Type III Effectors. Authors: Singer, A.U. / Wu, B. / Yee, A. / Houliston, S. / Xu, X. / Cui, H. / Skarina, T. / Garcia, M. / Semesi, A. / Arrowsmith, C.H. / Savchenko, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2lf6.cif.gz | 614.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2lf6.ent.gz | 517 KB | Display | PDB format |
PDBx/mmJSON format | 2lf6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lf/2lf6 ftp://data.pdbj.org/pub/pdb/validation_reports/lf/2lf6 | HTTPS FTP |
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-Related structure data
Related structure data | 2lf3C 3sviC 3tjyC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11275.709 Da / Num. of mol.: 1 / Fragment: sequence database residues 220-320 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas syringae pv. phaseolicola 1448A (bacteria) Strain: 1448A / Race 6 / Gene: hopAB1, virPphA, PSPPH_A0127 / Production host: Escherichia coli (E. coli) / References: UniProt: Q48B61 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 300 / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |