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Yorodumi- PDB-2cqq: Solution Structure of RSGI RUH-037, a myb DNA-binding domain in h... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2cqq | ||||||
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Title | Solution Structure of RSGI RUH-037, a myb DNA-binding domain in human cDNA | ||||||
Components | DnaJ homolog subfamily C member 1 | ||||||
Keywords | MEMBRANE PROTEIN / STRUCTURAL GENOMICS / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information regulation of protein secretion / ATPase activator activity / endomembrane system / negative regulation of proteolysis / protein folding / regulation of translation / protein-folding chaperone binding / nuclear membrane / endoplasmic reticulum membrane / endoplasmic reticulum ...regulation of protein secretion / ATPase activator activity / endomembrane system / negative regulation of proteolysis / protein folding / regulation of translation / protein-folding chaperone binding / nuclear membrane / endoplasmic reticulum membrane / endoplasmic reticulum / DNA binding / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Doi-Katayama, Y. / Hirota, H. / Hayashi, F. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution Structure of RSGI RUH-037, a myb DNA-binding domain in human cDNA Authors: Doi-Katayama, Y. / Hirota, H. / Hayashi, F. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2cqq.cif.gz | 398.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2cqq.ent.gz | 346.4 KB | Display | PDB format |
PDBx/mmJSON format | 2cqq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cq/2cqq ftp://data.pdbj.org/pub/pdb/validation_reports/cq/2cqq | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 7489.286 Da / Num. of mol.: 1 / Fragment: myb DNA-binding domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: Cell-free protein synthesis / Gene: AY225122 / Plasmid: P041115-03 / References: UniProt: Q96KC8 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.55mM myb DNA-binding domain U-15N, 13C; 20mM d-Tris-HCl; 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120mM NaCl / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function, structures with the lowest energy, structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |