+Open data
-Basic information
Entry | Database: PDB / ID: 2ks4 | ||||||
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Title | NMR structure of the sea anemone actinoporin Sticholysin | ||||||
Components | Sticholysin-1 | ||||||
Keywords | TRANSPORT PROTEIN / protein / Cytolysis / Hemolysis / Ion transport / Membrane / Nematocyst / Porin / Secreted / Toxin / Transmembrane / Transport | ||||||
Function / homology | Function and homology information : / nematocyst / pore complex assembly / other organism cell membrane / channel activity / pore complex / monoatomic cation transport / toxin activity / extracellular region Similarity search - Function | ||||||
Biological species | Stichodactyla helianthus (sea anemone) | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Authors | Castrillo, I. / Santoro, J. / Bruix, M. | ||||||
Citation | Journal: Biomol.Nmr Assign. / Year: 2009 Title: 1H, 13C, and 15N NMR assignments of the actinoporin Sticholysin I. Authors: Castrillo, I. / Alegre-Cebollada, J. / del Pozo, A.M. / Gavilanes, J.G. / Santoro, J. / Bruix, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ks4.cif.gz | 1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2ks4.ent.gz | 875.7 KB | Display | PDB format |
PDBx/mmJSON format | 2ks4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ks/2ks4 ftp://data.pdbj.org/pub/pdb/validation_reports/ks/2ks4 | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 19409.953 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Stichodactyla helianthus (sea anemone) / Gene: STCH1, STCH / Production host: Escherichia coli (E. coli) / Strain (production host): RB791 / References: UniProt: P81662 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | pH: 4.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AV800 / Manufacturer: Bruker / Model: AV800 / Field strength: 800.2 MHz |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 20 / Conformers submitted total number: 20 |