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- PDB-2j76: Solution structure and RNA interactions of the RNA recognition mo... -

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Basic information

Entry
Database: PDB / ID: 2j76
TitleSolution structure and RNA interactions of the RNA recognition motif from eukaryotic translation initiation factor 4B
ComponentsEUKARYOTIC TRANSLATION INITIATION FACTOR 4B
KeywordsTRANSLATION / PROTEIN BIOSYNTHESIS / RNA RECOGNITION MOTIF / INITIATION FACTOR / RNA BINDING DOMAIN / RRM / RBD / RNP / RNA-BINDING / PHOSPHORYLATION
Function / homology
Function and homology information


Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / eukaryotic translation initiation factor 4F complex / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / Deadenylation of mRNA / M-decay: degradation of maternal mRNAs by maternally stored factors / regulation of translational initiation / Ribosomal scanning and start codon recognition / Translation initiation complex formation / mTORC1-mediated signalling / GTP hydrolysis and joining of the 60S ribosomal subunit ...Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / eukaryotic translation initiation factor 4F complex / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / Deadenylation of mRNA / M-decay: degradation of maternal mRNAs by maternally stored factors / regulation of translational initiation / Ribosomal scanning and start codon recognition / Translation initiation complex formation / mTORC1-mediated signalling / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / translation initiation factor activity / RNA binding / cytosol
Similarity search - Function
Eukaryotic translation initiation factor 4B / RRM (RNA recognition motif) domain / RNA recognition motif / RNA recognition motif / Eukaryotic RNA Recognition Motif (RRM) profile. / RNA recognition motif domain / RNA-binding domain superfamily / Nucleotide-binding alpha-beta plait domain superfamily / Alpha-Beta Plaits / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Eukaryotic translation initiation factor 4B
Similarity search - Component
Biological speciesHOMO SAPIENS (human)
MethodSOLUTION NMR
Model type detailsMINIMIZED AVERAGE
AuthorsFleming, K. / Ghuman, J. / Yuan, X.M. / Simpson, P. / Szendroi, A. / Matthews, S. / Curry, S.
CitationJournal: Biochemistry / Year: 2003
Title: Solution Structure and RNA Interactions of the RNA Recognition Motif from Eukaryotic Translation Initiation Factor 4B.
Authors: Fleming, K. / Ghuman, J. / Yuan, X.M. / Simpson, P. / Szendroi, A. / Matthews, S. / Curry, S.
History
DepositionOct 6, 2006Deposition site: PDBE / Processing site: PDBE
Revision 1.0Oct 28, 2008Provider: repository / Type: Initial release
Revision 1.1Apr 19, 2017Group: Other
Revision 1.2May 15, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
E: EUKARYOTIC TRANSLATION INITIATION FACTOR 4B


Theoretical massNumber of molelcules
Total (without water)11,4581
Polymers11,4581
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / -
Representative

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Components

#1: Protein EUKARYOTIC TRANSLATION INITIATION FACTOR 4B / EIF-4B / EIF4B


Mass: 11457.733 Da / Num. of mol.: 1 / Fragment: RRM, RESIDUES 87-176 / Source method: obtained synthetically / Source: (synth.) HOMO SAPIENS (human) / References: UniProt: P23588

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR detailsText: NONE

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Sample preparation

Sample conditionsTemperature: 310.0 K

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NMR measurement

NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Bruker DRXBrukerDRX5001
Bruker DRXBrukerDRX8002

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Processing

NMR softwareName: X-PLOR / Version: 3.1
Developer: BRUNGER,ADAMS,CLORE,DELANO,GROS, GROSSE- KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,READ, RICE,SIMONSON,WARREN
Classification: refinement
RefinementSoftware ordinal: 1 / Details: REFINED WITH RDC MEASUREMENTS
NMR ensembleConformers submitted total number: 1

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