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Yorodumi- PDB-2i96: Solution structure of the oxidized microsomal human cytochrome b5 -
+Open data
-Basic information
Entry | Database: PDB / ID: 2i96 | ||||||
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Title | Solution structure of the oxidized microsomal human cytochrome b5 | ||||||
Components | Cytochrome b5 | ||||||
Keywords | ELECTRON TRANSPORT / b5 fold | ||||||
Function / homology | Function and homology information Vitamin C (ascorbate) metabolism / L-ascorbic acid metabolic process / cytochrome-c oxidase activity / Insertion of tail-anchored proteins into the endoplasmic reticulum membrane / response to cadmium ion / mitochondrial outer membrane / membrane => GO:0016020 / electron transfer activity / intracellular membrane-bounded organelle / heme binding ...Vitamin C (ascorbate) metabolism / L-ascorbic acid metabolic process / cytochrome-c oxidase activity / Insertion of tail-anchored proteins into the endoplasmic reticulum membrane / response to cadmium ion / mitochondrial outer membrane / membrane => GO:0016020 / electron transfer activity / intracellular membrane-bounded organelle / heme binding / endoplasmic reticulum membrane / enzyme binding / endoplasmic reticulum / membrane / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Nunez-Quintana, M. / Truan, G. / Van Heijenoort, C. | ||||||
Citation | Journal: To be Published Title: Solution Structure of human cytochrome b5 Authors: Nunez-Quintana, M. / Truan, G. / Van Heijenoort, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2i96.cif.gz | 775.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2i96.ent.gz | 656 KB | Display | PDB format |
PDBx/mmJSON format | 2i96.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i9/2i96 ftp://data.pdbj.org/pub/pdb/validation_reports/i9/2i96 | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12295.493 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: pET15b / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P00167 |
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#2: Chemical | ChemComp-HEM / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | pH: 6.5 / Pressure: 1 atm / Temperature: 308 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |