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Yorodumi- PDB-2i6m: Crystal structure of the complexes of the archaeal sulfolobus PTP... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2i6m | ||||||
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Title | Crystal structure of the complexes of the archaeal sulfolobus PTP-fold phosphatase with Tungstate | ||||||
Components | Sulfolobus solfataricus protein tyrosine phosphatase | ||||||
Keywords | HYDROLASE / PTP domain | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Sulfolobus solfataricus (archaea) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||
Authors | Chu, H.M. / Wang, A.H.J. | ||||||
Citation | Journal: Proteins / Year: 2006 Title: Enzyme-substrate interactions revealed by the crystal structures of the archaeal Sulfolobus PTP-fold phosphatase and its phosphopeptide complexes Authors: Chu, H.M. / Wang, A.H.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2i6m.cif.gz | 44.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2i6m.ent.gz | 31.1 KB | Display | PDB format |
PDBx/mmJSON format | 2i6m.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i6/2i6m ftp://data.pdbj.org/pub/pdb/validation_reports/i6/2i6m | HTTPS FTP |
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-Related structure data
Related structure data | 2dxpC 2i6iC 2i6jC 2i6oC 2i6pC 1oheS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 18433.164 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sulfolobus solfataricus (archaea) / Plasmid: pET21 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q97VZ7, protein-tyrosine-phosphatase |
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#2: Chemical | ChemComp-WO4 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.61 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.54 Å |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Jun 30, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→50 Å / Num. all: 13524 / Num. obs: 11220 / % possible obs: 83 % / Observed criterion σ(F): 0 / Redundancy: 2.4 % / Rmerge(I) obs: 0.09 / Rsym value: 0.064 / Net I/σ(I): 12.6 |
Reflection shell | Resolution: 1.9→1.97 Å / Redundancy: 2.3 % / Rmerge(I) obs: 0.555 / Mean I/σ(I) obs: 1.72 / Num. unique all: 956 / Rsym value: 0.49 / % possible all: 73.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1OHE Resolution: 1.9→50 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 1.9→50 Å
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Refine LS restraints |
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