+Open data
-Basic information
Entry | Database: PDB / ID: 2i30 | ||||||
---|---|---|---|---|---|---|---|
Title | Human serum albumin complexed with myristate and salicylic acid | ||||||
Components | Serum albumin | ||||||
Keywords | LIPID BINDING PROTEIN / PLASMA PROTEIN / METAL-BINDING / LIPID-BINDING | ||||||
Function / homology | Function and homology information cellular response to calcium ion starvation / exogenous protein binding / Ciprofloxacin ADME / enterobactin binding / Heme biosynthesis / HDL remodeling / negative regulation of mitochondrial depolarization / Prednisone ADME / Heme degradation / Aspirin ADME ...cellular response to calcium ion starvation / exogenous protein binding / Ciprofloxacin ADME / enterobactin binding / Heme biosynthesis / HDL remodeling / negative regulation of mitochondrial depolarization / Prednisone ADME / Heme degradation / Aspirin ADME / antioxidant activity / toxic substance binding / Scavenging of heme from plasma / Recycling of bile acids and salts / cellular response to starvation / platelet alpha granule lumen / fatty acid binding / Post-translational protein phosphorylation / Cytoprotection by HMOX1 / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / pyridoxal phosphate binding / Platelet degranulation / protein-folding chaperone binding / blood microparticle / copper ion binding / endoplasmic reticulum lumen / Golgi apparatus / endoplasmic reticulum / protein-containing complex / DNA binding / extracellular space / extracellular exosome / extracellular region / identical protein binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.9 Å | ||||||
Authors | Yang, F. / Bian, C. / Zhu, L. / Zhao, G. / Huang, Z. / Huang, M. | ||||||
Citation | Journal: J.Struct.Biol. / Year: 2007 Title: Effect of human serum albumin on drug metabolism: Structural evidence of esterase activity of human serum albumin Authors: Yang, F. / Bian, C. / Zhu, L. / Zhao, G. / Huang, Z. / Huang, M. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2i30.cif.gz | 130.1 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2i30.ent.gz | 100.9 KB | Display | PDB format |
PDBx/mmJSON format | 2i30.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i3/2i30 ftp://data.pdbj.org/pub/pdb/validation_reports/i3/2i30 | HTTPS FTP |
---|
-Related structure data
Related structure data | 2i2zC 1bj5S C: citing same article (ref.) S: Starting model for refinement |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 66571.219 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P02768 | ||
---|---|---|---|
#2: Chemical | #3: Chemical | ChemComp-MYR / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.52 Å3/Da / Density % sol: 51.24 % |
---|---|
Crystal grow | Temperature: 293 K / Method: evaporation, vapor diffusion, sitting drop / pH: 7.5 Details: 31% PEG 3350, 0.05M potassium phosphate, pH 7.5, EVAPORATION, vapor diffusion, sitting drop, temperature 293K |
-Data collection
Diffraction | Mean temperature: 293 K |
---|---|
Diffraction source | Source: ROTATING ANODE / Type: ENRAF-NONIUS FR591 / Wavelength: 1.5418 / Wavelength: 1.5418 Å |
Detector | Type: BRUKER SMART 2000 / Detector: CCD / Date: Aug 20, 2004 |
Radiation | Monochromator: MIRROR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→45.7 Å / Num. all: 15230 / Num. obs: 13859 / % possible obs: 91 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 6.38 |
Reflection shell | Resolution: 2.9→3.08 Å / % possible all: 88.7 |
-Processing
Software |
| ||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1BJ5 Resolution: 2.9→45.7 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
| ||||||||||||||||||||||||||||
Solvent computation | Bsol: 62.534 Å2 | ||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 60.788 Å2
| ||||||||||||||||||||||||||||
Refine analyze |
| ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→45.7 Å
| ||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.9→3.08 Å / Rfactor Rfree error: 0.011
| ||||||||||||||||||||||||||||
Xplor file |
|