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- PDB-2fxi: Arsenate reductase (ArsC from pI258) C10S/C15A double mutant with... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2fxi | ||||||
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Title | Arsenate reductase (ArsC from pI258) C10S/C15A double mutant with sulfate in its active site | ||||||
![]() | Protein arsC | ||||||
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Function / homology | ![]() arsenate reductase (thioredoxin) / arsenate reductase (thioredoxin) activity / response to arsenic-containing substance / ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Loris, R. / Buts, L. / Messens, J. | ||||||
![]() | ![]() Title: Interplay Between Ion Binding and Catalysis in the Thioredoxin-coupled Arsenate Reductase Family. Authors: Roos, G. / Buts, L. / Van Belle, K. / Brosens, E. / Geerlings, P. / Loris, R. / Wyns, L. / Messens, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 43.7 KB | Display | ![]() |
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PDB format | ![]() | 29.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2cd7C ![]() 1ljlS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 14783.608 Da / Num. of mol.: 1 / Mutation: C10S,C15A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() References: UniProt: P0A006, EC: 1.20.4.-, ![]() |
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#2: Chemical | ChemComp-K / |
#3: Chemical | ChemComp-SO4 / ![]() |
#4: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 35.23 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 100 mM TRIS ph 8.0, 10 mM DTT, 100 mM KCl, 10 mM K2SO4, 42.5% PEG-4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Mar 3, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.8→20 Å / Num. all: 10780 / Num. obs: 10780 / % possible obs: 97.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.3 % / Biso Wilson estimate: 12.6 Å2 / Rmerge(I) obs: 0.037 / Rsym value: 0.037 / Net I/σ(I): 15.5 |
Reflection shell | Resolution: 1.8→1.86 Å / Redundancy: 2.3 % / Rmerge(I) obs: 0.08 / Mean I/σ(I) obs: 6.6 / Num. unique all: 914 / Rsym value: 0.08 / % possible all: 83.9 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: pdb entry 1ljl Resolution: 1.8→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.8→20 Å
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Refine LS restraints |
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