+Open data
-Basic information
Entry | Database: PDB / ID: 2fvf | ||||||
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Title | Structure of 10:0-ACP (protein with docked fatty acid) | ||||||
Components | Acyl Carrier Protein | ||||||
Keywords | BIOSYNTHETIC PROTEIN / 4-helix bundle | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Spinacia oleracea (spinach) | ||||||
Method | SOLUTION NMR / Initial structure from torsion angle dynamics (in CYANA). Fatty acid, phosphopantetheine were added, docked using ambiguous distance restraints in XPLOR-NIH | ||||||
Authors | Zornetzer, G.A. / Fox, B.G. / Markley, J.L. | ||||||
Citation | Journal: Biochemistry / Year: 2006 Title: Solution structures of spinach acyl carrier protein with decanoate and stearate Authors: Zornetzer, G.A. / Fox, B.G. / Markley, J.L. | ||||||
History |
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Remark 999 | SEQUENCE The sequence of the protein has 100% match to the sequence from GB entry CAA31207 (ACP-I ...SEQUENCE The sequence of the protein has 100% match to the sequence from GB entry CAA31207 (ACP-I polypeptide, synthetic construct). |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2fvf.cif.gz | 503.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2fvf.ent.gz | 440.6 KB | Display | PDB format |
PDBx/mmJSON format | 2fvf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fv/2fvf ftp://data.pdbj.org/pub/pdb/validation_reports/fv/2fvf | HTTPS FTP |
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-Related structure data
Related structure data | 2avaC 2fvaC 2fveC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8844.879 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spinacia oleracea (spinach) / Plasmid: pSACP-2t / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P07854 |
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#2: Chemical | ChemComp-PNS / |
#3: Chemical | ChemComp-DKA / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 2 mM protein, 10 mM MES pH 6.1, 100 mM NaCl, 95% H2O/5% D2O Solvent system: 95% H2O/5% D2O |
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Sample conditions | Ionic strength: 100 mM NaCl / pH: 6.1 / Pressure: 1 atm / Temperature: 287 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: Initial structure from torsion angle dynamics (in CYANA). Fatty acid, phosphopantetheine were added, docked using ambiguous distance restraints in XPLOR-NIH Software ordinal: 1 | ||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with lowest energy in a clustering of the fatty acid structures Conformers calculated total number: 200 / Conformers submitted total number: 20 |