+Open data
-Basic information
Entry | Database: PDB / ID: 2fmc | ||||||
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Title | Solution structure of the class I hydrophobin EAS | ||||||
Components | Hydrophobin | ||||||
Keywords | SURFACE ACTIVE PROTEIN / beta barrel / flexible loop / disulphide bonds | ||||||
Function / homology | Function and homology information structural constituent of cell wall / fungal-type cell wall / cell wall organization / extracellular region Similarity search - Function | ||||||
Biological species | Neurospora crassa (fungus) | ||||||
Method | SOLUTION NMR / simulated annealing, molecular dynamics, torsion angle dynamics | ||||||
Authors | Kwan, A.H. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.Usa / Year: 2006 Title: Structural basis for rodlet assembly in fungal hydrophobins Authors: Kwan, A.H. / Winefield, R.D. / Sunde, M. / Matthews, J.M. / Haverkamp, R.G. / Templeton, M.D. / Mackay, J.P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2fmc.cif.gz | 432.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2fmc.ent.gz | 377.2 KB | Display | PDB format |
PDBx/mmJSON format | 2fmc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fm/2fmc ftp://data.pdbj.org/pub/pdb/validation_reports/fm/2fmc | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8189.339 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Neurospora crassa (fungus) / Tissue: spores / References: UniProt: Q04571 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: Other standard triple resonance NMR expts were also collected, including: HNCO, HNCA, HNCACB, CBCA(CO)NH, HCCH-TOCSY etc |
-Sample preparation
Details |
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Sample conditions |
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-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, molecular dynamics, torsion angle dynamics Software ordinal: 1 Details: the structures are based on: 1623 NOE-derived distance constraints, 90 dihedral angle restraints,10 distance restraints from hydrogen bonds. | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 300 / Conformers submitted total number: 20 |