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Yorodumi- PDB-2ez0: Crystal structure of the S107A/E148Q/Y445A mutant of EcClC, in co... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2ez0 | ||||||
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Title | Crystal structure of the S107A/E148Q/Y445A mutant of EcClC, in complex with a FaB fragment | ||||||
Components |
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Keywords | MEMBRANE PROTEIN / ClC family of channels and transporters / H+/Cl- antiporter / membrane protein-Fab complex | ||||||
Function / homology | Function and homology information chloride:proton antiporter activity / cellular stress response to acidic pH / voltage-gated chloride channel activity / chloride transmembrane transport / proton transmembrane transport / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.54 Å | ||||||
Authors | Lobet, S. / Dutzler, R. | ||||||
Citation | Journal: Embo J. / Year: 2006 Title: Ion-binding properties of the ClC chloride selectivity filter. Authors: Lobet, S. / Dutzler, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ez0.cif.gz | 336.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ez0.ent.gz | 271.4 KB | Display | PDB format |
PDBx/mmJSON format | 2ez0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ez/2ez0 ftp://data.pdbj.org/pub/pdb/validation_reports/ez/2ez0 | HTTPS FTP |
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-Related structure data
Related structure data | 2exwC 2exyC 1otuS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 50281.324 Da / Num. of mol.: 2 / Mutation: S107A, E148Q, Y445A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Plasmid: pET28 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 DE3 / References: UniProt: P37019 #2: Antibody | Mass: 23823.031 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Cell line: HYBRIDOMA CELL LINE #3: Antibody | Mass: 23088.443 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Cell line: HYBRIDOMA CELL LINE #4: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.13 Å3/Da / Density % sol: 70.22 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 9.5 Details: 36% peg200/300 1:2, 100mM NaBr, 50 mM NaKTart, 50 mM Glycine, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 0.91931 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Feb 14, 2005 |
Radiation | Monochromator: LN2 cooled fixed-exit Si(111) monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.91931 Å / Relative weight: 1 |
Reflection | Resolution: 3.5→50 Å / Num. all: 37589 / Num. obs: 33492 / % possible obs: 89.1 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Biso Wilson estimate: 72.6 Å2 |
Reflection shell | Resolution: 3.5→3.62 Å / % possible all: 90.6 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: pdb entry 1OTU Resolution: 3.54→19.92 Å / Rfactor Rfree error: 0.007 / Data cutoff high absF: 4326688.23 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 14.3666 Å2 / ksol: 0.220033 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 91 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.54→19.92 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.5→3.72 Å / Rfactor Rfree error: 0.03 / Total num. of bins used: 6
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Xplor file |
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