+Open data
-Basic information
Entry | Database: PDB / ID: 2ek8 | ||||||
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Title | Aminopeptidase from Aneurinibacillus sp. strain AM-1 | ||||||
Components | Aminopeptidase | ||||||
Keywords | HYDROLASE / METALLOPROTEINASE | ||||||
Function / homology | Function and homology information metalloexopeptidase activity / aminopeptidase activity / proteolysis / metal ion binding Similarity search - Function | ||||||
Biological species | Aneurinibacillus sp. (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 1.8 Å | ||||||
Authors | Akioka, M. / Nakano, H. / Watanabe, K. | ||||||
Citation | Journal: To be published Title: Structural characterization of a novel bacterial aminopeptidase with an apical domain from aneurinibacillus sp. strain AM-1 Authors: Akioka, M. / Nakano, H. / Tsujimoto, Y. / Matsui, H. / Nakatsu, T. / Kato, H. / Watanabe, K. #1: Journal: ACTA CRYSTALLOGR.,SECT.F / Year: 2006 Title: Overexpression, purification, crystallization and preliminary X-ray crystallographic studies of a proline-specific aminopeptidase from Aneurinibacillus sp. strain AM-1 Authors: Akioka, M. / Nakano, H. / Horikiri, A. / Tsujimoto, Y. / Matsui, H. / Shimizu, T. / Nakatsu, T. / Kato, H. / Watanabe, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ek8.cif.gz | 103.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ek8.ent.gz | 78.1 KB | Display | PDB format |
PDBx/mmJSON format | 2ek8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ek/2ek8 ftp://data.pdbj.org/pub/pdb/validation_reports/ek/2ek8 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 45289.699 Da / Num. of mol.: 1 / Fragment: Residues 1-421 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aneurinibacillus sp. (bacteria) / Strain: AM-1 / Plasmid: PET-11A / Production host: Escherichia coli (E. coli) / References: UniProt: A2V759 | ||||
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#2: Chemical | ChemComp-ZN / #3: Chemical | ChemComp-IPA / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 4 |
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-Sample preparation
Crystal | Density Matthews: 2.72 Å3/Da / Density % sol: 54.85 % |
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Crystal grow | Temperature: 299 K / Method: vapor diffusion / pH: 5.8 Details: 13% PEG 8000, 0.1M MES-NaOH, 0.2M Zinc acetate, pH 5.8, VAPOR DIFFUSION, temperature 299K |
-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.8→19.9 Å / Num. obs: 45919 / % possible obs: 98.7 % / Redundancy: 10.6 % / Biso Wilson estimate: 18.5 Å2 / Rmerge(I) obs: 0.058 / Net I/σ(I): 31.2 | ||||||||||||||||||
Reflection shell | Resolution: 1.8→1.9 Å / Redundancy: 10.5 % / Rmerge(I) obs: 0.336 / Mean I/σ(I) obs: 6.1 / % possible all: 97.1 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 1.8→19.85 Å / Rfactor Rfree error: 0.005 / Data cutoff high absF: 1875354.05 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 46.2604 Å2 / ksol: 0.37918 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.1 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.8→19.85 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.91 Å / Rfactor Rfree error: 0.013 / Total num. of bins used: 6
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Xplor file |
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