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Yorodumi- PDB-2ck0: ANTI-ANTI-IDIOTYPIC ANTIBODY AGAINST HUMAN ANGIOTENSIN II, COMPLE... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2ck0 | ||||||
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Title | ANTI-ANTI-IDIOTYPIC ANTIBODY AGAINST HUMAN ANGIOTENSIN II, COMPLEX WITH A SYNTHETIC CYCLIC PEPTIDE | ||||||
Components |
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Keywords | IMMUNOGLOBULIN | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Mus musculus (house mouse) synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.2 Å | ||||||
Authors | Pan, Y.H. / Amzel, L.M. | ||||||
Citation | Journal: To be Published Title: Structures of Angiotensin II and a Phage-Display Selected Cyclic Peptide in Complex with Fab131: Making Angiotensin II Analogs Authors: Pan, Y.H. / Lee, K.H. / Li, M. / Ronco, P. / Amzel, L.M. #1: Journal: To be Published Title: Structures of Angiotensin II and a Phage-Display Selected Cyclic Peptide in Complex with Fab131: Making Angiotensin II Analogs Authors: Pan, Y.H. / Lee, K.H. / Li, M. / Ronco, P. / Amzel, L.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ck0.cif.gz | 96.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ck0.ent.gz | 73.9 KB | Display | PDB format |
PDBx/mmJSON format | 2ck0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ck/2ck0 ftp://data.pdbj.org/pub/pdb/validation_reports/ck/2ck0 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Antibody | Mass: 23904.543 Da / Num. of mol.: 1 / Fragment: FAB FRAGMENT / Source method: isolated from a natural source / Details: IGG1, KAPPA / Source: (natural) Mus musculus (house mouse) / Cell: HYBRIDOMA / References: GenBank: 1407830, UniProt: P01837*PLUS |
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#2: Antibody | Mass: 23650.486 Da / Num. of mol.: 1 / Fragment: FAB FRAGMENT / Source method: isolated from a natural source / Details: IGG1, KAPPA / Source: (natural) Mus musculus (house mouse) / Cell: HYBRIDOMA |
#3: Protein/peptide | Mass: 1195.388 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: CYCLIC PEPTIDE, SYNTHETIC / Source: (natural) synthetic construct (others) |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.52 Å3/Da / Density % sol: 51 % |
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Crystal grow | pH: 7.5 / Details: pH 7.5 |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: RIGAKU / Detector: IMAGE PLATE / Details: MONOCHROMATOR |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→100 Å / Num. obs: 24870 / % possible obs: 90 % / Observed criterion σ(I): 0 / Redundancy: 6.2 % / Rsym value: 0.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.2→6 Å / Data cutoff low absF: 0.1 / Cross valid method: THROUGHOUT / σ(F): 3
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Displacement parameters | Biso mean: 30.9 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→6 Å
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Refine LS restraints |
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Xplor file |
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