+Open data
-Basic information
Entry | Database: PDB / ID: 2b0g | ||||||
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Title | Solution Structure of Drosophila melanogaster SNF RBD2 | ||||||
Components | U1 small nuclear ribonucleoprotein A | ||||||
Keywords | RNA BINDING PROTEIN / SNF RBD2 / RNA binding / RNA splicing / solution structure | ||||||
Function / homology | Function and homology information primary sex determination, soma / snRNA stem-loop binding / pre-mRNA intronic pyrimidine-rich binding / mRNA Splicing - Major Pathway / female germ-line sex determination / small nuclear ribonucleoprotein complex / poly(U) RNA binding / U2 snRNP / U1 snRNP / oogenesis ...primary sex determination, soma / snRNA stem-loop binding / pre-mRNA intronic pyrimidine-rich binding / mRNA Splicing - Major Pathway / female germ-line sex determination / small nuclear ribonucleoprotein complex / poly(U) RNA binding / U2 snRNP / U1 snRNP / oogenesis / precatalytic spliceosome / regulation of alternative mRNA splicing, via spliceosome / U2 snRNA binding / U1 snRNA binding / catalytic step 2 spliceosome / RNA splicing / spliceosomal complex / mRNA splicing, via spliceosome / ribonucleoprotein complex / mRNA binding / protein-containing complex / identical protein binding / nucleus Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | SOLUTION NMR / Simulated annealing, molecular dynamics | ||||||
Model type details | minimized average | ||||||
Authors | Cui, G. / Li, C. / Jin, C. / Xia, B. | ||||||
Citation | Journal: TO BE PUBLISHED Title: Solution Structure of Drosophila melanogaster SNF RBD2 Authors: Cui, G. / Li, C. / Jin, C. / Xia, B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2b0g.cif.gz | 35.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2b0g.ent.gz | 25 KB | Display | PDB format |
PDBx/mmJSON format | 2b0g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b0/2b0g ftp://data.pdbj.org/pub/pdb/validation_reports/b0/2b0g | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9519.945 Da / Num. of mol.: 1 / Fragment: SNF RBD2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Plasmid: pET-28(a+) / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta(DE3)pLysS / References: UniProt: P43332 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: This structure is the averaged structure of SNF RBD2 and energy-minimized by AMBER |
-Sample preparation
Details | Contents: 1mM SNF RBD2 U-15N, 13C; 50mM phosphate buffer NA; 1mM EDTA; 50mM NaCl; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 250 mM / pH: 7.2 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: Simulated annealing, molecular dynamics / Software ordinal: 1 Details: The structure was based on total 3828 restraints.3715 are NOE derived distance restraints, 61 are hihedral angle restraints, 52 are distance restraints from hydrogen bonds. | ||||||||||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||||||||||
NMR ensemble | Conformers submitted total number: 1 |