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- PDB-2azv: Solution structure of the T22G mutant of N-terminal SH3 domain of... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2azv | ||||||
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Title | Solution structure of the T22G mutant of N-terminal SH3 domain of DRK (calculated without NOEs) | ||||||
![]() | SH2-SH3 adapter protein drk | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Bezsonova, I. / Singer, A.U. / Choy, W.-Y. / Tollinger, M. / Forman-Kay, J.D. | ||||||
![]() | ![]() Title: Structural Comparison of the Unstable drkN SH3 Domain and a Stable Mutant Authors: Bezsonova, I. / Singer, A. / Choy, W.-Y. / Tollinger, M. / Forman-Kay, J.D. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 188.3 KB | Display | ![]() |
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PDB format | ![]() | 154.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2a36C ![]() 2a37C ![]() 2azsC C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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NMR ensembles |
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Components
#1: Protein | Mass: 6824.576 Da / Num. of mol.: 1 / Fragment: N-terminal SH3 domain, residues 1-59 / Mutation: T22G Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() | ||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: T22G DRKN SH3 DOMAIN 15N, 13C, 50mM PHOSPHATE BUFFER, 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 50 mM / pH: 6.0 / Pressure: 1 atm / Temperature: 293 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model![]() |
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Processing
NMR software |
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Refinement | Method: ![]() Details: THE STRUCTURES ARE BASED ON RESIDUAL DIPOLAR COUPLING RESTRAINTS,CARBONYL CHEMICAL SHIFT ANISOTROPY RESTRAINTS, DIHEDRAL ANGLE RESTRAINTS AND HYDROGEN BOND RESTRAINTS | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 |