+Open data
-Basic information
Entry | Database: PDB / ID: 2a36 | ||||||
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Title | Solution structure of the N-terminal SH3 domain of DRK | ||||||
Components | Protein E(sev)2B | ||||||
Keywords | SIGNALING PROTEIN / DROSOPHILA MELANOGASTER / SH3 FRAGMENT / DRK | ||||||
Function / homology | Function and homology information sevenless binding / PI3K Cascade / Downstream signal transduction / Generation of second messenger molecules / : / PI-3K cascade:FGFR1 / PI-3K cascade:FGFR2 / PI-3K cascade:FGFR3 / PI-3K cascade:FGFR4 / RET signaling ...sevenless binding / PI3K Cascade / Downstream signal transduction / Generation of second messenger molecules / : / PI-3K cascade:FGFR1 / PI-3K cascade:FGFR2 / PI-3K cascade:FGFR3 / PI-3K cascade:FGFR4 / RET signaling / Erythropoietin activates RAS / FLT3 Signaling / SOS-mediated signalling / Signaling by SCF-KIT / Regulation of KIT signaling / Signalling to RAS / : / DAP12 signaling / SHC-related events triggered by IGF1R / Role of LAT2/NTAL/LAB on calcium mobilization / FCERI mediated Ca+2 mobilization / SHC-mediated cascade:FGFR1 / FRS-mediated FGFR1 signaling / SHC-mediated cascade:FGFR2 / FRS-mediated FGFR2 signaling / SHC-mediated cascade:FGFR3 / FRS-mediated FGFR3 signaling / FRS-mediated FGFR4 signaling / SHC-mediated cascade:FGFR4 / Signal attenuation / Insulin receptor signalling cascade / FCERI mediated MAPK activation / PIP3 activates AKT signaling / GAB1 signalosome / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / SHC1 events in ERBB4 signaling / GRB2 events in EGFR signaling / SHC1 events in EGFR signaling / GRB2 events in ERBB2 signaling / EGFR Transactivation by Gastrin / RAF/MAP kinase cascade / torso signaling pathway / tracheal outgrowth, open tracheal system / R7 cell fate commitment / Spry regulation of FGF signaling / Negative regulation of FGFR1 signaling / Negative regulation of FGFR2 signaling / Negative regulation of FGFR3 signaling / Negative regulation of FGFR4 signaling / Regulation of signaling by CBL / sevenless signaling pathway / RHOU GTPase cycle / EGFR downregulation / Cargo recognition for clathrin-mediated endocytosis / RHO GTPases Activate WASPs and WAVEs / Regulation of actin dynamics for phagocytic cup formation / epithelial cell migration, open tracheal system / follicle cell of egg chamber development / Clathrin-mediated endocytosis / imaginal disc-derived wing morphogenesis / olfactory learning / short-term memory / COP9 signalosome / epidermal growth factor receptor binding / regulation of MAPK cascade / associative learning / positive regulation of cell size / fibroblast growth factor receptor signaling pathway / vascular endothelial growth factor receptor signaling pathway / positive regulation of TORC1 signaling / phosphotyrosine residue binding / sensory perception of sound / epidermal growth factor receptor signaling pathway / positive regulation of receptor-mediated endocytosis / signaling receptor complex adaptor activity / presynapse / insulin receptor signaling pathway / Ras protein signal transduction / positive regulation of ERK1 and ERK2 cascade / signal transduction / protein-containing complex / nucleoplasm / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Forman-Kay, J.D. / Bezsonova, I. / Singer, A. / Choy, W.-Y. / Tollinger, M. | ||||||
Citation | Journal: Biochemistry / Year: 2005 Title: Structural Comparison of the Unstable drkN SH3 Domain and a Stable Mutant Authors: Bezsonova, I. / Singer, A. / Choy, W.-Y. / Tollinger, M. / Forman-Kay, J.D. | ||||||
History |
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Remark 400 | COMPOUND RESIDUE ANI PRESENT IN THE CONSTRAINT FILE DEFINES THE ALIGNMENT FRAME. INCLUDE THESE ...COMPOUND RESIDUE ANI PRESENT IN THE CONSTRAINT FILE DEFINES THE ALIGNMENT FRAME. INCLUDE THESE COORDINATES TO USE THE RESIDUAL DIPOLAR COUPLING AND/OR CARBONYL CHEMICAL SHIFT RESTRAINTS |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2a36.cif.gz | 189.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2a36.ent.gz | 154.9 KB | Display | PDB format |
PDBx/mmJSON format | 2a36.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a3/2a36 ftp://data.pdbj.org/pub/pdb/validation_reports/a3/2a36 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6868.628 Da / Num. of mol.: 1 / Fragment: N-TERMINAL SH3 DOMAIN, RESIDUES 1-59 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: drk, E(sev)2B / Plasmid: PET-11A / Production host: Escherichia coli (E. coli) / Strain (production host): HMS174 / References: UniProt: Q08012 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: HNCO-based 3D experiments were used for measuring 15N-1HN, 15N-13C', 13C'-13CA RDCs and C'-CSA restraints.13CA-13CB and 13CA-1HA RDCs were also measured. |
-Sample preparation
Details | Contents: 1MM DRKN SH3 DOMAIN 15N,13C, 500 MM NA2SO4, 50 MM PHOSPHATE BUFFER, 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 500mM / pH: 6.0 / Pressure: 1 atm / Temperature: 293 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 500 MHz |
-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 |