+Open data
-Basic information
Entry | Database: PDB / ID: 1y90 | ||||||||||||||||||
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Title | HIV-1 Dis(Mal) Duplex Mn-Soaked | ||||||||||||||||||
Components | 5'-R(*Keywords | RNA / HIV-1 / metal ions / bulge | Function / homology | : / RNA / RNA (> 10) | Function and homology information Method | X-RAY DIFFRACTION / FOURIER SYNTHESIS / Resolution: 3.08 Å | Authors | Ennifar, E. / Walter, P. / Dumas, P. | Citation | Journal: Nucleic Acids Res. / Year: 2003 Title: A crystallographic study of the binding of 13 metal ions to two related RNA duplexes Authors: Ennifar, E. / Walter, P. / Dumas, P. #1: Journal: Structure Fold.Des. / Year: 1999 Title: The crystal structure of the dimerization initiation site of genomic HIV-1 RNA reveals an extended duplex with two adenine bulges Authors: Ennifar, E. / Yusupov, M. / Walter, P. / Marquet, R. / Ehresmann, B. / Ehresmann, C. / Dumas, P. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1y90.cif.gz | 37.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1y90.ent.gz | 26 KB | Display | PDB format |
PDBx/mmJSON format | 1y90.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/y9/1y90 ftp://data.pdbj.org/pub/pdb/validation_reports/y9/1y90 | HTTPS FTP |
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-Related structure data
Related structure data | 1nlcC 1o3zC 1wvdC 1y6sC 1y6tC 1y73C 1y95C 2oijC C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: RNA chain | Mass: 7481.368 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: HIV-1 DIS RNA #2: Chemical | ChemComp-MN / |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 50 % | ||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 310 K / Method: vapor diffusion, sitting drop / pH: 7 Details: MPD, spermine, KCl, MgCl2, Na Cacodylate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 310K | ||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 120 K |
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Diffraction source | Source: ROTATING ANODE / Type: ENRAF-NONIUS / Wavelength: 1.54 Å |
Detector | Type: MACSCIENCE / Detector: IMAGE PLATE |
Radiation | Monochromator: mirror / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 3.08→15 Å / Num. obs: 2446 / % possible obs: 96.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3 % / Rsym value: 0.052 |
Reflection shell | Highest resolution: 3.08 Å / Rsym value: 0.112 / % possible all: 92.1 |
-Processing
Software |
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Refinement | Method to determine structure: FOURIER SYNTHESIS / Resolution: 3.08→10 Å / Rfactor Rfree error: 0.018 / Data cutoff high absF: 439218.88 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 16.3725 Å2 / ksol: 0.262868 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 47 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.08→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.08→3.27 Å / Rfactor Rfree error: 0.047 / Total num. of bins used: 6
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Xplor file |
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