+Open data
-Basic information
Entry | Database: PDB / ID: 1wwz | ||||||
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Title | Crystal structure of PH1933 from Pyrococcus horikoshii OT3 | ||||||
Components | hypothetical protein PH1933Hypothesis | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / Pyrococcus horikoshii OT3 / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Pyrococcus horikoshii (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 1.75 Å | ||||||
Authors | Asada, Y. / Kunishima, N. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Crystal structure of PH1933 from Pyrococcus horikoshii OT3 Authors: Asada, Y. / Kunishima, N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wwz.cif.gz | 85.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wwz.ent.gz | 65.1 KB | Display | PDB format |
PDBx/mmJSON format | 1wwz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ww/1wwz ftp://data.pdbj.org/pub/pdb/validation_reports/ww/1wwz | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The biological assembly is dimer |
-Components
#1: Protein | Mass: 18494.596 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pyrococcus horikoshii (archaea) / Strain: OT3 / Plasmid: pET11a / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: O59596 #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50.81 % |
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Crystal grow | Temperature: 291 K / Method: microbatch / pH: 7.5 Details: HEPES-Na, iso-Propanol, PEG 4000, Glycerol, Acetyl-CoA, pH 7.5, MICROBATCH, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL26B1 / Wavelength: 1.0, 0.97907 | |||||||||
Detector | Type: RIGAKU RAXIS V / Detector: IMAGE PLATE / Date: Mar 11, 2004 / Details: mirrors | |||||||||
Radiation | Monochromator: mirrors / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 1.75→30 Å / Num. all: 38152 / Num. obs: 38152 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 5.9 % / Biso Wilson estimate: 21.745 Å2 / Rmerge(I) obs: 0.081 / Rsym value: 0.075 / Net I/σ(I): 10 | |||||||||
Reflection shell | Resolution: 1.75→1.81 Å / Redundancy: 5.5 % / Rmerge(I) obs: 0.419 / Mean I/σ(I) obs: 4.11 / Num. unique all: 3724 / Rsym value: 0.378 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 1.75→28.28 Å / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 25.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.75→28.28 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.75→1.83 Å / Rfactor Rfree error: 0.018
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