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Open data
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Basic information
Entry | Database: PDB / ID: 1wvv | ||||||
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Title | Crystal structure of chitinase C mutant E147Q | ||||||
![]() | chitinase C | ||||||
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Function / homology | ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kezuka, Y. / Watanabe, T. / Nonaka, T. | ||||||
![]() | ![]() Title: Structural Studies of a Two-domain Chitinase from Streptomyces griseus HUT6037 Authors: Kezuka, Y. / Ohishi, M. / Itoh, Y. / Watanabe, J. / Mitsutomi, M. / Watanabe, T. / Nonaka, T. #1: Journal: Biosci.Biotechnol.Biochem. / Year: 2002 Title: Functional analysis of the chitin-binding domain of a family 19 chitinase from Streptomyces griseus HUT6037: substrate-binding affinity and cis-dominant increase of antifungal function Authors: Itoh, Y. / Kawase, T. / Nikaidou, N. / Fukada, H. / Mitsutomi, M. / Watanabe, T. / Itoh, Y. #2: Journal: Microbiology / Year: 1999 Title: Family 19 chitinases of Streptomyces species: characterization and distribution Authors: Watanabe, T. / Kanai, R. / Kawase, T. / Tanabe, T. / Mitsutomi, M. / Sakuda, S. / Miyashita, K. #3: Journal: J.Bacteriol. / Year: 1996 Title: A modular family 19 chitinase found in the prokaryotic organism Streptomyces griseus HUT 6037 Authors: Ohno, T. / Armand, S. / Hata, T. / Nikaidou, N. / Henrissat, B. / Mitsutomi, M. / Watanabe, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 105.7 KB | Display | ![]() |
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PDB format | ![]() | 85.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 28496.182 Da / Num. of mol.: 2 / Fragment: residues 30-294 / Mutation: E147Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: ![]() ![]() #2: Chemical | ChemComp-CL / ![]() #3: Chemical | ![]() #4: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.1 Å3/Da / Density % sol: 59.5 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4.6 Details: Sodium chloride, Sodium acetate buffer, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Oct 22, 2003 |
Radiation | Monochromator: Triangular Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2→56.79 Å / Num. all: 47236 / Num. obs: 47236 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 20.9 % / Biso Wilson estimate: 30.337 Å2 / Rmerge(I) obs: 0.062 / Rsym value: 0.062 / Net I/σ(I): 6.9 |
Reflection shell | Resolution: 2→2.05 Å / Redundancy: 13.6 % / Rmerge(I) obs: 0.242 / Mean I/σ(I) obs: 3 / Num. unique all: 3466 / Rsym value: 0.242 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 27.077 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→56.8 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.052 Å / Total num. of bins used: 20
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