+Open data
-Basic information
Entry | Database: PDB / ID: 1wou | ||||||
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Title | Crystal Structure of human Trp14 | ||||||
Components | thioredoxin -related protein, 14 kDa | ||||||
Keywords | ELECTRON TRANSPORT | ||||||
Function / homology | Function and homology information protein-disulfide reductase (NAD(P)H) activity / tumor necrosis factor-mediated signaling pathway / peroxidase activity / extracellular exosome / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MAD / Resolution: 1.8 Å | ||||||
Authors | Woo, J.R. / Kim, S.J. / Jeong, W. / Cho, Y.H. / Lee, S.C. / Chung, Y.J. / Rhee, S.G. / Ryu, S.E. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2004 Title: Structural basis of cellular redox regulation by human TRP14 Authors: Woo, J.R. / Kim, S.J. / Jeong, W. / Cho, Y.H. / Lee, S.C. / Chung, Y.J. / Rhee, S.G. / Ryu, S.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wou.cif.gz | 29.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wou.ent.gz | 23.2 KB | Display | PDB format |
PDBx/mmJSON format | 1wou.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wo/1wou ftp://data.pdbj.org/pub/pdb/validation_reports/wo/1wou | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 13957.786 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: pET11a / Production host: Escherichia coli (E. coli) / References: UniProt: Q9BRA2 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 2 |
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-Sample preparation
Crystal | Density Matthews: 1.97 Å3/Da / Density % sol: 37 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: polyethylene glycol 8000, polyethylene glycol monomethylether 2000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU300 / Wavelength: 1.5418 Å | ||||||||||||||||||
Detector |
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Radiation |
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Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 | ||||||||||||||||||
Reflection | Resolution: 1.8→40 Å / Num. all: 10425 / Num. obs: 10385 / % possible obs: 99.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.041 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 1.8→22.57 Å / Isotropic thermal model: isothermal / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.8→22.57 Å
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