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Yorodumi- PDB-1wj5: Solution structure of the hypothetical domain of RIKEN cDNA 0610009H20 -
+Open data
-Basic information
Entry | Database: PDB / ID: 1wj5 | ||||||
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Title | Solution structure of the hypothetical domain of RIKEN cDNA 0610009H20 | ||||||
Components | hypothetical protein (RIKEN cDNA 0610009H20)Hypothesis | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / Winged Helix / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information Polymerase switching on the C-strand of the telomere / Telomere C-strand synthesis initiation / CST complex / telomere maintenance via telomere lengthening / single-stranded telomeric DNA binding / telomere capping / intermediate filament cytoskeleton / telomeric DNA binding / negative regulation of telomere maintenance via telomerase / telomere maintenance ...Polymerase switching on the C-strand of the telomere / Telomere C-strand synthesis initiation / CST complex / telomere maintenance via telomere lengthening / single-stranded telomeric DNA binding / telomere capping / intermediate filament cytoskeleton / telomeric DNA binding / negative regulation of telomere maintenance via telomerase / telomere maintenance / positive regulation of DNA replication / fibrillar center / single-stranded DNA binding / chromosome, telomeric region / intracellular membrane-bounded organelle / nucleoplasm / nucleus Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR | ||||||
Authors | Hatta, R. / Hayashi, F. / Yoshida, M. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution structure of the hypothetical domain of RIKEN cDNA 0610009H20 Authors: Hatta, R. / Hayashi, F. / Yoshida, M. / Yokoyama, S. | ||||||
History |
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Remark 700 | sheet author determination method: author determined |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wj5.cif.gz | 710.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wj5.ent.gz | 592.9 KB | Display | PDB format |
PDBx/mmJSON format | 1wj5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wj/1wj5 ftp://data.pdbj.org/pub/pdb/validation_reports/wj/1wj5 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13049.385 Da / Num. of mol.: 1 / Fragment: hypothetical domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Description: Cell-free protein synthesis / Gene: FANTOM 2 cDNA 0610009H20 / Plasmid: P030811-04 / References: UniProt: Q8K2X3 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.05mM 13C, 15N-labeled protein; 20mM d-Tris-HCl; 100mM NaCl; 1mM DTT; 0.02% NaN3; 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120 / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
-Processing
NMR software |
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NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations, target function Conformers calculated total number: 100 / Conformers submitted total number: 20 |