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Yorodumi- PDB-1wih: Solution structure of RSGI RUH-021, a domain II of ribosome recyc... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1wih | ||||||
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Title | Solution structure of RSGI RUH-021, a domain II of ribosome recycling factor from mouse cDNA | ||||||
Components | mitochondrial ribosome recycling factor | ||||||
Keywords | Structural genomics / unknown function / ribosome recycling factor / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information Mitochondrial translation termination / ribosome disassembly / ribosomal large subunit binding / translation / mitochondrion Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / simulated annealing, torsion angle dynamics | ||||||
Authors | Abe, T. / Hirota, H. / Koshiba, S. / Kigawa, T. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution structure of RSGI RUH-021, a domain II of ribosome recycling factor from Mouse cDNA Authors: Abe, T. / Hirota, H. / Koshiba, S. / Kigawa, T. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wih.cif.gz | 463.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wih.ent.gz | 404.1 KB | Display | PDB format |
PDBx/mmJSON format | 1wih.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wi/1wih ftp://data.pdbj.org/pub/pdb/validation_reports/wi/1wih | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8539.772 Da / Num. of mol.: 1 / Fragment: domain II Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Tissue: tongue / Description: Cell-free protein synthesis system / Gene: RIKEN cDNA 2310061P21 / Plasmid: P030908-28 / References: UniProt: Q9D6S7 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy. |
-Sample preparation
Details | Contents: 0.50mM RSGI RUH-021 U-15N, 13C; 20mM d-Tris-HCl buffer; 100mM NaCl; 2mM d-DTT; 0.02% NaN3; 10% D20 Solvent system: 90% H2O, 10% D20 |
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Sample conditions | Ionic strength: 100mM / pH: 7.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: simulated annealing, torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy and target function | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations, structures with the lowest energy, target function Conformers calculated total number: 100 / Conformers submitted total number: 20 |