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Yorodumi- PDB-1w2e: The Crystal Structure of the Bacterial Cell Division Protein ZapA -
+Open data
-Basic information
Entry | Database: PDB / ID: 1w2e | ||||||
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Title | The Crystal Structure of the Bacterial Cell Division Protein ZapA | ||||||
Components | ZAPA | ||||||
Keywords | BACTERIAL CELL DIVISION / FTSZ MODULATOR | ||||||
Function / homology | Function and homology information septin ring assembly / cell septum / FtsZ-dependent cytokinesis / division septum assembly / cell division site / cytosol Similarity search - Function | ||||||
Biological species | PSEUDOMONAS AERUGINOSA (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.8 Å | ||||||
Authors | Low, H.H. / Moncrieffe, M.C. / Lowe, J. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2004 Title: The Crystal Structure of Zapa and its Modulation of Ftsz Polymerisation Authors: Low, H.H. / Moncrieffe, M.C. / Lowe, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1w2e.cif.gz | 43.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1w2e.ent.gz | 34.7 KB | Display | PDB format |
PDBx/mmJSON format | 1w2e.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/w2/1w2e ftp://data.pdbj.org/pub/pdb/validation_reports/w2/1w2e | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 11813.772 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) PSEUDOMONAS AERUGINOSA (bacteria) / Strain: PA01-LAC / Plasmid: PHIS17 / Production host: ESCHERICHIA COLI BL21(DE3) (bacteria) / Variant (production host): C41 / References: UniProt: Q9HTW3 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.15 Å3/Da / Density % sol: 43 % |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX14.2 / Wavelength: 0.97 |
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→74.33 Å / Num. obs: 5317 / % possible obs: 99.5 % / Observed criterion σ(I): 0 / Redundancy: 12.1 % / Biso Wilson estimate: 65.699 Å2 / Rmerge(I) obs: 0.04 / Net I/σ(I): 47.5 |
Reflection shell | Resolution: 2.8→2.95 Å / Redundancy: 12.6 % / Rmerge(I) obs: 0.06 / Mean I/σ(I) obs: 31.5 / % possible all: 99.5 |
-Processing
Software | Name: CNS / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: MAD / Resolution: 2.8→74.33 Å / Data cutoff high absF: 10000 / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Bsol: 39.4988 Å2 / ksol: 0.357469 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 53.711 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→74.33 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.8→2.93 Å / Total num. of bins used: 8
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Xplor file |
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