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Yorodumi- PDB-1vhh: A POTENTIAL CATALYTIC SITE WITHIN THE AMINO-TERMINAL SIGNALLING D... -
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-Basic information
Entry | Database: PDB / ID: 1vhh | ||||||
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Title | A POTENTIAL CATALYTIC SITE WITHIN THE AMINO-TERMINAL SIGNALLING DOMAIN OF SONIC HEDGEHOG | ||||||
Components | SONIC HEDGEHOGSonic hedgehog protein | ||||||
Keywords | SIGNALLING PROTEIN | ||||||
Function / homology | Function and homology information forebrain regionalization / cell proliferation in external granule layer / zona limitans intrathalamica formation / positive regulation of neurotrophin production / positive regulation of photoreceptor cell differentiation / fungiform papilla development / Release of Hh-Np from the secreting cell / digestive tract mesoderm development / epithelial-mesenchymal signaling involved in prostate gland development / fungiform papilla morphogenesis ...forebrain regionalization / cell proliferation in external granule layer / zona limitans intrathalamica formation / positive regulation of neurotrophin production / positive regulation of photoreceptor cell differentiation / fungiform papilla development / Release of Hh-Np from the secreting cell / digestive tract mesoderm development / epithelial-mesenchymal signaling involved in prostate gland development / fungiform papilla morphogenesis / ventral spinal cord interneuron specification / tongue morphogenesis / respiratory tube development / Ligand-receptor interactions / Activation of SMO / mesenchymal-epithelial cell signaling involved in prostate gland development / positive regulation of oligodendrocyte progenitor proliferation / trachea development / positive regulation of skeletal muscle cell proliferation / anatomical structure formation involved in morphogenesis / right lung development / left lung development / primary prostatic bud elongation / regulation of mesenchymal cell proliferation involved in prostate gland development / mesenchymal smoothened signaling pathway involved in prostate gland development / positive regulation of sclerotome development / tracheoesophageal septum formation / negative regulation of ureter smooth muscle cell differentiation / positive regulation of ureter smooth muscle cell differentiation / negative regulation of kidney smooth muscle cell differentiation / positive regulation of kidney smooth muscle cell differentiation / regulation of odontogenesis / positive regulation of mesenchymal cell proliferation involved in ureter development / polarity specification of anterior/posterior axis / trunk neural crest cell migration / hindgut morphogenesis / striated muscle tissue development / negative regulation of alpha-beta T cell differentiation / regulation of glial cell proliferation / regulation of prostatic bud formation / positive regulation of penile erection / metanephric mesenchymal cell proliferation involved in metanephros development / neural tube formation / formation of anatomical boundary / lung epithelium development / positive regulation of striated muscle cell differentiation / myotube differentiation / trachea morphogenesis / regulation of epithelial cell proliferation involved in prostate gland development / cholesterol-protein transferase activity / bud outgrowth involved in lung branching / telencephalon regionalization / epithelial-mesenchymal cell signaling / : / laminin-1 binding / vasculogenesis involved in coronary vascular morphogenesis / Hedgehog ligand biogenesis / salivary gland cavitation / negative regulation of cholesterol efflux / determination of left/right asymmetry in lateral mesoderm / spinal cord dorsal/ventral patterning / negative regulation of mesenchymal cell apoptotic process / positive regulation of cerebellar granule cell precursor proliferation / cell development / negative regulation of T cell differentiation in thymus / spinal cord motor neuron differentiation / positive regulation of T cell differentiation in thymus / cerebellar granule cell precursor proliferation / intermediate filament organization / mesenchymal cell apoptotic process / embryonic skeletal system development / male genitalia morphogenesis / limb bud formation / lung lobe morphogenesis / prostate gland development / skeletal muscle fiber differentiation / establishment of epithelial cell polarity / fungiform papilla formation / patched binding / embryonic foregut morphogenesis / thalamus development / embryonic digestive tract morphogenesis / somite development / hindbrain development / positive regulation of skeletal muscle tissue development / epithelial cell proliferation involved in salivary gland morphogenesis / ectoderm development / animal organ formation / neuron fate commitment / dorsal/ventral neural tube patterning / stem cell development / branching involved in prostate gland morphogenesis / mesenchymal cell proliferation involved in lung development / negative regulation of dopaminergic neuron differentiation / negative thymic T cell selection / skeletal muscle cell proliferation / positive regulation of immature T cell proliferation in thymus / oligodendrocyte development / mesenchymal cell proliferation / lymphoid progenitor cell differentiation Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.7 Å | ||||||
Authors | Hall, T.M.T. / Porter, J.A. / Beachy, P.A. / Leahy, D.J. | ||||||
Citation | Journal: Nature / Year: 1995 Title: A potential catalytic site revealed by the 1.7-A crystal structure of the amino-terminal signalling domain of Sonic hedgehog. Authors: Hall, T.M. / Porter, J.A. / Beachy, P.A. / Leahy, D.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1vhh.cif.gz | 56.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1vhh.ent.gz | 41 KB | Display | PDB format |
PDBx/mmJSON format | 1vhh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vh/1vhh ftp://data.pdbj.org/pub/pdb/validation_reports/vh/1vhh | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 50 |
-Components
#1: Protein | Mass: 18544.869 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: POTENTIAL / Plasmid: PGEX2T / Production host: Escherichia coli (E. coli) / References: UniProt: Q62226 |
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#2: Chemical | ChemComp-ZN / |
#3: Chemical | ChemComp-SO4 / |
#4: Water | ChemComp-HOH / |
Source details | THE AMINO-TERMINAL DOMAIN OF MURINE SONIC HEDGEHOG WAS EXPRESSED AS A GLUTATHIONE S-TRANSFERASE ...THE AMINO-TERMINAL DOMAIN OF MURINE SONIC HEDGEHOG WAS EXPRESSED AS A GLUTATHION |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39.22 % | ||||||||||||||||||||||||||||||
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Crystal | *PLUS Density % sol: 35 % | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 6.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X4A / Wavelength: 0.9686, 0.9792, 0.9793, 0.9879 | |||||||||||||||
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Detector | Type: FUJI / Detector: IMAGE PLATE / Date: Jan 6, 1995 | |||||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.7→30 Å / Num. obs: 14968 / % possible obs: 86.4 % / Redundancy: 6.7 % / Rmerge(I) obs: 0.08 | |||||||||||||||
Reflection | *PLUS Rmerge(I) obs: 0.08 |
-Processing
Software |
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Refinement | Resolution: 1.7→6 Å / σ(F): 2
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Displacement parameters | Biso mean: 10.53 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→6 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor all: 0.202 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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