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Yorodumi- PDB-1v10: Structure of Rigidoporus lignosus laccase from hemihedrally twinn... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1v10 | ||||||
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Title | Structure of Rigidoporus lignosus laccase from hemihedrally twinned crystals | ||||||
Components | LACCASE | ||||||
Keywords | OXIDASE / MULTICOPPER BLUE OXIDASE | ||||||
Function / homology | Function and homology information hydroquinone:oxygen oxidoreductase activity / laccase / copper ion binding / extracellular region Similarity search - Function | ||||||
Biological species | RIGIDOPORUS LIGNOSUS (fungus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.7 Å | ||||||
Authors | Rizzi, M. / Garavaglia, S. / Palmieri, F. / Cambria, A. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2004 Title: The Structure of Rigidoporus Lignosus Laccase Containing a Full Complement of Copper Ions, Reveals an Asymmetrical Arrangement for the T3 Copper Pair Authors: Garavaglia, S. / Cambria, M.T. / Miglio, M. / Ragusa, S. / Iacobazzi, V. / Palmieri, F. / D'Ambrosio, C. / Scaloni, A. / Rizzi, M. #1: Journal: Protein Expr.Purif. / Year: 2000 Title: Production, Purification, and Properties of an Extracellular Laccase from Rigidoporus Lignosus Authors: Cambria, M.T. / Cambria, A. / Ragusa, S. / Rizzarelli, E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1v10.cif.gz | 109.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1v10.ent.gz | 81.5 KB | Display | PDB format |
PDBx/mmJSON format | 1v10.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/v1/1v10 ftp://data.pdbj.org/pub/pdb/validation_reports/v1/1v10 | HTTPS FTP |
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-Related structure data
Related structure data | 1gycS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 55603.805 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) RIGIDOPORUS LIGNOSUS (fungus) / References: UniProt: Q6H9H7*PLUS, laccase | ||
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#2: Chemical | ChemComp-CU / #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.56 Å3/Da / Density % sol: 66 % |
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Crystal grow | pH: 6.5 / Details: pH 6.50 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-1 / Wavelength: 0.93 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.93 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→20 Å / Num. obs: 91011 / % possible obs: 99 % / Observed criterion σ(I): 3 / Redundancy: 2.9 % / Rmerge(I) obs: 0.057 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1GYC Resolution: 1.7→20 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 1.7→20 Å
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Refine LS restraints |
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