+Open data
-Basic information
Entry | Database: PDB / ID: 1v5y | ||||||
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Title | Binding of coumarins to NAD(P)H:FMN oxidoreductase | ||||||
Components | Major NAD(P)H-flavin oxidoreductase | ||||||
Keywords | OXIDOREDUCTASE / coumarin / FMN oxidoreductase / inhibitor | ||||||
Function / homology | Function and homology information Oxidoreductases; Acting on NADH or NADPH; With unknown physiological acceptors / bioluminescence / oxidoreductase activity Similarity search - Function | ||||||
Biological species | Aliivibrio fischeri (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 1.9 Å | ||||||
Authors | Kobori, T. / Koike, H. / Sasaki, H. / Zenno, S. / Saigo, K. / Tanokura, M. | ||||||
Citation | Journal: To be Published Title: Binding of coumarins to NAD(P)H:FMN oxidoreductase Authors: Kobori, T. / Koike, H. / Sasaki, H. / Zenno, S. / Saigo, K. / Tanokura, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1v5y.cif.gz | 101.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1v5y.ent.gz | 78.4 KB | Display | PDB format |
PDBx/mmJSON format | 1v5y.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/v5/1v5y ftp://data.pdbj.org/pub/pdb/validation_reports/v5/1v5y | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 24624.775 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aliivibrio fischeri (bacteria) / Gene: FRaseI / Production host: Escherichia coli (E. coli) References: UniProt: P46072, Oxidoreductases; Acting on NADH or NADPH; With unknown physiological acceptors #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.37 Å3/Da / Density % sol: 47.64 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: PEG 4000, sodium acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction |
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Diffraction source |
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Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
-Processing
Software |
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Refinement | Method to determine structure: FOURIER SYNTHESIS / Resolution: 1.9→10 Å / Rfactor Rfree: 0.226 / Rfactor Rwork: 0.185 / Rfactor obs: 0.185 | ||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→10 Å
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