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Yorodumi- PDB-1tu1: Crystal Structure of Protein of Unknown Function PA94 from Pseudo... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1tu1 | ||||||
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Title | Crystal Structure of Protein of Unknown Function PA94 from Pseudomonas aeruginosa, Putative Regulator | ||||||
Components | hypothetical protein PA0094Hypothesis | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / hypothetical protein / PA0094 / PSI / Protein Structure Initiative / Midwest Center for Structural Genomics / MCSG | ||||||
Function / homology | Inner membrane lipoprotein DcrB/EagT6 / DcrB / Mog1/PsbP, alpha/beta/alpha sandwich / Protein Transport Mog1p; Chain A / Mog1/PsbP, alpha/beta/alpha sandwich / 3-Layer(aba) Sandwich / Alpha Beta / DI(HYDROXYETHYL)ETHER / Effector EagT6 Function and homology information | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 1.95 Å | ||||||
Authors | Osipiuk, J. / Evdokimova, E. / Savchenko, A. / Edwards, A. / Cymborowski, M. / Minor, W. / Joachimiak, A. / Midwest Center for Structural Genomics (MCSG) | ||||||
Citation | Journal: To be Published Title: X-ray crystal structure of hypothetical protein PA94 from Pseudomonas aeruginosa Authors: Osipiuk, J. / Evdokimova, E. / Savchenko, A. / Edwards, A. / Cymborowski, M. / Minor, W. / Joachimiak, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1tu1.cif.gz | 79.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1tu1.ent.gz | 60 KB | Display | PDB format |
PDBx/mmJSON format | 1tu1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tu/1tu1 ftp://data.pdbj.org/pub/pdb/validation_reports/tu/1tu1 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | the biological assembly is unknown |
-Components
#1: Protein | Mass: 16849.057 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Strain: PAO1 / Gene: PA0094 / Plasmid: pET15b / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q9I738 #2: Chemical | #3: Chemical | ChemComp-PEG / #4: Chemical | ChemComp-SO4 / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.1 Å3/Da / Density % sol: 60 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: Ammonium sulphate, PEG 400, HEPES buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97934 Å |
Detector | Type: SBC-2 / Detector: CCD / Date: Mar 24, 2004 |
Radiation | Monochromator: double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97934 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→40 Å / Num. obs: 31380 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 9.6 % / Rmerge(I) obs: 0.052 / Net I/σ(I): 38.1 |
Reflection shell | Resolution: 1.95→2.01 Å / Redundancy: 7.2 % / Rmerge(I) obs: 0.478 / Mean I/σ(I) obs: 3.52 / Num. unique all: 2529 / % possible all: 98.2 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 1.95→40 Å / Cor.coef. Fo:Fc: 0.949 / SU B: 2.748 / SU ML: 0.078 / σ(F): 0 / σ(I): 0 / ESU R: 0.147 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.423 Å2
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Refinement step | Cycle: LAST / Resolution: 1.95→40 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.95→2.001 Å / Total num. of bins used: 20
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