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- PDB-1tqm: Crystal Structure of A. fulgidus Rio2 Serine Protein Kinase Bound... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1tqm | ||||||
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Title | Crystal Structure of A. fulgidus Rio2 Serine Protein Kinase Bound to AMPPNP | ||||||
![]() | conserved hypothetical protein | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | LaRonde-LeBlanc, N. / Wlodawer, A. | ||||||
![]() | ![]() Title: Crystal Structure of A. fulgidus Rio2 Defines a New Family of Serine Protein Kinases Authors: LaRonde-LeBlanc, N. / Wlodawer, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 74 KB | Display | ![]() |
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PDB format | ![]() | 53.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1tqiSC ![]() 1tqpC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 32857.645 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-ANP / |
#3: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.57 Å3/Da / Density % sol: 51.67 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 3.83 Details: PEG 900, SODIUM PHOSPHATE, SODIUM CITRATE, pH 3.83, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Nov 21, 2003 |
Radiation | Monochromator: SAGITALLY FOCUSED Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.99→29 Å / Num. all: 21420 / Num. obs: 20313 / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 7.5 % / Rsym value: 0.042 / Net I/σ(I): 40.9 |
Reflection shell | Resolution: 1.99→2.04 Å / Redundancy: 6.8 % / Mean I/σ(I) obs: 12.1 / Rsym value: 0.125 / % possible all: 95.7 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: pdb entry 1tqi Resolution: 1.99→28.99 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.933 / SU B: 3.353 / SU ML: 0.097 / Isotropic thermal model: ISOTROPIC / Cross valid method: THROUGHOUT / ESU R: 0.166 / ESU R Free: 0.16 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.56 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.99→28.99 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.99→2.042 Å / Total num. of bins used: 20
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