+Open data
-Basic information
Entry | Database: PDB / ID: 1t66 | ||||||
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Title | The structure of FAB with intermediate affinity for fluorescein. | ||||||
Components |
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Keywords | IMMUNE SYSTEM / Antibody / Fab / Fluorescein | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Terzyan, S. / Ramsland, P.A. / Voss Jr., E.W. / Herron, J.N. / Edmundson, A.B. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2004 Title: Three-dimensional Structures of Idiotypically Related Fabs with Intermediate and High Affinity for Fluorescein. Authors: Terzyan, S. / Ramsland, P.A. / Voss Jr., E.W. / Herron, J.N. / Edmundson, A.B. | ||||||
History |
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Remark 900 | SEQUENCE THE SEQUENCE REFERENCES ARE: E. A. KABAT, T. T. WU, M. REID-MILLER, H. M. PERRY, K. S. ...SEQUENCE THE SEQUENCE REFERENCES ARE: E. A. KABAT, T. T. WU, M. REID-MILLER, H. M. PERRY, K. S. GOTTESMAN, SEQUENCES OF PROTEINS OF IMMUNOLOGICAL INTEREST, U.S. PHS. W. D. BEDZYK, L. S. JOHNSON, G. S. RIORDAN, E. W. VOSS JR. (1989), COMPARISON OF VARIABLE REGION PRIMARY STRUCTURES WITHIN AN ANTI-FLUORESCINE IDIOTYPE FAMILY, J. BIOL. CHEM. V. 264, 1565-1569. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1t66.cif.gz | 183.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1t66.ent.gz | 146.6 KB | Display | PDB format |
PDBx/mmJSON format | 1t66.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/t6/1t66 ftp://data.pdbj.org/pub/pdb/validation_reports/t6/1t66 | HTTPS FTP |
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-Related structure data
Related structure data | 1flrS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Antibody | Mass: 24167.814 Da / Num. of mol.: 2 / Fragment: FAB / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Cell: LYMPHOCYTE-PLASMA CELL / Cell line: 9-40 murine-murine hybridoma / Organ: spleen / Variant: BALB/CV / Strain: BALB/C / References: GenBank: 18044164, UniProt: Q65ZC0*PLUS #2: Antibody | Mass: 24027.107 Da / Num. of mol.: 2 / Fragment: FAB / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Cell: LYMPHOCYTE-PLASMA CELL / Cell line: 9-40 murine-murine hybridoma / Organ: spleen / Variant: BALB/CV / Strain: BALB/C / References: UniProt: Q91Z05*PLUS #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 50 % |
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Crystal grow | Temperature: 286 K / pH: 6.2 Details: ammonium sulphate, sodium phosphate, pH 6.2, batch, temperature 286K, pH 6.20 |
-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU300 / Wavelength: 1.54 |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Details: OSMIC MAXFLUX |
Radiation | Monochromator: OSMIC MAXFLUX / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→47.1 Å / Num. obs: 49354 / % possible obs: 97.6 % / Observed criterion σ(I): -3 / Redundancy: 4.2 % / Biso Wilson estimate: 41.7 Å2 / Rmerge(I) obs: 0.104 / Net I/σ(I): 8.8 |
Reflection shell | Resolution: 2.3→2.38 Å / Redundancy: 2.2 % / Rmerge(I) obs: 0.533 / Mean I/σ(I) obs: 1.8 / % possible all: 95.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1FLR Resolution: 2.3→47 Å / Isotropic thermal model: ANISOTROPIC OVERALL B-FACTOR / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: ENGH & HUBER
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Solvent computation | Solvent model: CNSL / Bsol: 42.59 Å2 / ksol: 0.312 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 43.2 Å2
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Refinement step | Cycle: LAST / Resolution: 2.3→47 Å
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Refine LS restraints |
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