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Yorodumi- PDB-1t2y: NMR solution structure of the protein part of Cu6-Neurospora crassa MT -
+Open data
-Basic information
Entry | Database: PDB / ID: 1t2y | ||||||
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Title | NMR solution structure of the protein part of Cu6-Neurospora crassa MT | ||||||
Components | Metallothionein | ||||||
Keywords | METAL BINDING PROTEIN / protein fold / no secondary structural elements | ||||||
Function / homology | Metallothionein domain superfamily / metal ion binding / Metallothionein Function and homology information | ||||||
Method | SOLUTION NMR / the hybrid distance geometry-dynamical simulated annealing protocol | ||||||
Authors | Cobine, P.A. / McKay, R.T. / Zangger, K. / Dameron, C.T. / Armitage, I.M. | ||||||
Citation | Journal: Eur.J.Biochem. / Year: 2004 Title: Solution structure of Cu metallothionein from the fungus Neurospora crassa Authors: Cobine, P.A. / McKay, R.T. / Zangger, K. / Dameron, C.T. / Armitage, I.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1t2y.cif.gz | 11 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1t2y.ent.gz | 7.1 KB | Display | PDB format |
PDBx/mmJSON format | 1t2y.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1t2y_validation.pdf.gz | 238.5 KB | Display | wwPDB validaton report |
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Full document | 1t2y_full_validation.pdf.gz | 238.3 KB | Display | |
Data in XML | 1t2y_validation.xml.gz | 2.1 KB | Display | |
Data in CIF | 1t2y_validation.cif.gz | 2.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/t2/1t2y ftp://data.pdbj.org/pub/pdb/validation_reports/t2/1t2y | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 2235.421 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: The peptide was chemically synthesized. The sequence of the peptide occurs naturally in the fungus neurospora crassa. References: UniProt: P02807 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques |
-Sample preparation
Details | Contents: 0.5 mM Neurospora crassa MT, 20 mM phosphate buffer, 0.1 mM 2,2-dimethyl-2-silapentane-5-sulfonate, 0.02% NaN3, 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 20 mM KPi / pH: 6.5 / Pressure: ambient / Temperature: 283 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
-Processing
NMR software |
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Refinement | Method: the hybrid distance geometry-dynamical simulated annealing protocol Software ordinal: 1 Details: the structure was calculated with 152 NOEs and 13 dihedral angle restraints | ||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||
NMR ensemble | Conformers submitted total number: 1 |