+Open data
-Basic information
Entry | Database: PDB / ID: 1t0k | ||||||||||||
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Title | Joint X-ray and NMR Refinement of Yeast L30e-mRNA complex | ||||||||||||
Components |
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Keywords | RIBOSOME / Joint NMR and X-ray refinement / Ribosomal Protein L30e / MBP fusion protein | ||||||||||||
Function / homology | Function and homology information pre-mRNA 5'-splice site binding / detection of maltose stimulus / maltose binding / maltose transport complex / maltose transport / maltodextrin transmembrane transport / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) ...pre-mRNA 5'-splice site binding / detection of maltose stimulus / maltose binding / maltose transport complex / maltose transport / maltodextrin transmembrane transport / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / L13a-mediated translational silencing of Ceruloplasmin expression / carbohydrate transmembrane transporter activity / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / carbohydrate transport / ATP-binding cassette (ABC) transporter complex / cell chemotaxis / rRNA processing / outer membrane-bounded periplasmic space / cytoplasmic translation / cytosolic large ribosomal subunit / periplasmic space / structural constituent of ribosome / DNA damage response / RNA binding / membrane / cytosol / cytoplasm Similarity search - Function | ||||||||||||
Biological species | Escherichia coli (E. coli) Saccharomyces cerevisiae (brewer's yeast) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.24 Å | ||||||||||||
Authors | Chao, J.A. / Williamson, J.R. | ||||||||||||
Citation | Journal: Structure / Year: 2004 Title: Joint X-Ray and NMR Refinement of the Yeast L30e-mRNA Complex Authors: Chao, J.A. / Williamson, J.R. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1t0k.cif.gz | 105.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1t0k.ent.gz | 82.6 KB | Display | PDB format |
PDBx/mmJSON format | 1t0k.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/t0/1t0k ftp://data.pdbj.org/pub/pdb/validation_reports/t0/1t0k | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: RNA chain | Mass: 4172.541 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: RNA chain | Mass: 5216.183 Da / Num. of mol.: 1 / Source method: obtained synthetically |
#3: Protein | Mass: 41770.188 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: MALE, B4034, Z5632, ECS5017 / Production host: Escherichia coli (E. coli) / References: UniProt: P02928, UniProt: P0AEX9*PLUS |
#4: Protein | Mass: 11430.364 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: see remark 400, COMPOUND Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL30, RPL32, YGL030W / Production host: Escherichia coli (E. coli) / References: UniProt: P14120 |
#5: Polysaccharide | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose / alpha-maltotetraose |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.57 Å3/Da / Density % sol: 73.1 % | ||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6 Details: Lithium Sulfate, Sodium Cacodylate, Magnesium Chloride, Jeffamine, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 295K | ||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 93 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL9-2 / Wavelength: 0.9795 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jun 6, 2001 |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 3.24→26 Å / Num. all: 18636 / Num. obs: 18636 / % possible obs: 98.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 2 |
Reflection shell | Resolution: 3.24→3.41 Å / % possible all: 97.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.24→26 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 3.24→26 Å
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Refine LS restraints |
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