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Yorodumi- PDB-1szy: Solution structure of ITALY1 ("Initiator tRNA Anticodon Loop from... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1szy | ||||||||||||||||||
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Title | Solution structure of ITALY1 ("Initiator tRNA Anticodon Loop from Yeast"), an unmodified 21-nt RNA with the sequence of the anticodon stem-loop of yeast initiator tRNA | ||||||||||||||||||
Components | 5'-R(P*Keywords | RNA / Initiator tRNA Anticodon Loop | Function / homology | RNA / RNA (> 10) | Function and homology information Method | SOLUTION NMR / Distance geometry, simulated annealing | Authors | Schweisguth, D.C. / Moore, P.B. | Citation | Journal: J.Mol.Biol. / Year: 1997 | Title: On the conformation of the anticodon loops of initiator and elongator methionine tRNAs. Authors: Schweisguth, D.C. / Moore, P.B. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1szy.cif.gz | 21.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1szy.ent.gz | 14.2 KB | Display | PDB format |
PDBx/mmJSON format | 1szy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sz/1szy ftp://data.pdbj.org/pub/pdb/validation_reports/sz/1szy | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 6703.052 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: See DCS dissertation p. 61. |
-Sample preparation
Details | Contents: S1 NMR buffer; 100mM KCl, 50mM NaCl, 4mM Na cacodylate, 0.2mM EDTA |
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Sample conditions | pH: 6.5 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: Distance geometry, simulated annealing / Software ordinal: 1 Details: RNA topology and parameters from Rife and Moore, NAR, 1996. Constraints in loop included 128 NOEs, 7 chi dihedrals inferred from NOEs, 20 v0-v4 ribose dihedrals, 8 a and z P dihedrals, 12 b ...Details: RNA topology and parameters from Rife and Moore, NAR, 1996. Constraints in loop included 128 NOEs, 7 chi dihedrals inferred from NOEs, 20 v0-v4 ribose dihedrals, 8 a and z P dihedrals, 12 b and g P dihedrals and 7 e P dihedrals. Stem was constrained to A-form based on qualitative spectral analysis. | ||||||||||||
NMR ensemble | Conformers submitted total number: 1 |