+Open data
-Basic information
Entry | Database: PDB / ID: 1syo | |||||||||
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Title | N-terminal 3 domains of CI-MPR bound to mannose 6-phosphate | |||||||||
Components | cation-independent mannose 6-phosphate receptor | |||||||||
Keywords | PROTEIN TRANSPORT / SUGAR BINDING PROTEIN / lectin / receptor / mannose 6-phosphate | |||||||||
Function / homology | Function and homology information kringle domain binding / insulin-like growth factor binding / lysosomal transport / D-mannose binding / endocytic vesicle / phosphoprotein binding / trans-Golgi network / late endosome / signaling receptor activity / endosome membrane ...kringle domain binding / insulin-like growth factor binding / lysosomal transport / D-mannose binding / endocytic vesicle / phosphoprotein binding / trans-Golgi network / late endosome / signaling receptor activity / endosome membrane / Golgi membrane / Golgi apparatus / cell surface / plasma membrane Similarity search - Function | |||||||||
Biological species | Bos taurus (cattle) | |||||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.2 Å | |||||||||
Authors | Olson, L.J. / Dahms, N.M. / Kim, J.-J.P. | |||||||||
Citation | Journal: J.Biol.Chem. / Year: 2004 Title: The N-terminal carbohydrate recognition site of the cation-independent mannose 6-phosphate receptor Authors: Olson, L.J. / Dahms, N.M. / Kim, J.-J.P. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1syo.cif.gz | 185.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1syo.ent.gz | 144.7 KB | Display | PDB format |
PDBx/mmJSON format | 1syo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sy/1syo ftp://data.pdbj.org/pub/pdb/validation_reports/sy/1syo | HTTPS FTP |
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-Related structure data
Related structure data | 1sz0C 1q25S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 47618.461 Da / Num. of mol.: 2 / Fragment: N-terminal 3 domains Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bos taurus (cattle) / Gene: IGF2R, M6P / Cell line (production host): 5B1-4 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: P08169 |
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-Sugars , 3 types, 6 molecules
#2: Polysaccharide | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-alpha- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose Source method: isolated from a genetically manipulated source | ||
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#3: Sugar | #4: Sugar | |
-Non-polymers , 2 types, 338 molecules
#5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.26 Å3/Da / Density % sol: 45.1 % |
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Crystal grow | Temperature: 292 K / pH: 6.35 Details: 25% PEG 4000, 0.1M sodium cacodylate (pH 6.35), 150mM NaCl, 50 mM imidazole (pH 6.7), 10 mM manganese chloride, 5mM beta-glycerophosphate, 10 mM mannose 6-phosphate, vapor diffusion, sitting ...Details: 25% PEG 4000, 0.1M sodium cacodylate (pH 6.35), 150mM NaCl, 50 mM imidazole (pH 6.7), 10 mM manganese chloride, 5mM beta-glycerophosphate, 10 mM mannose 6-phosphate, vapor diffusion, sitting drop, temperature 292K |
-Data collection
Diffraction | Mean temperature: 103 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: May 14, 2000 / Details: CONFOCAL BLUE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→29.96 Å / Num. obs: 38820 / % possible obs: 88.8 % / Observed criterion σ(I): 0 / Biso Wilson estimate: 12.7 Å2 / Rmerge(I) obs: 0.064 |
Reflection shell | Resolution: 2.2→2.28 Å / Rmerge(I) obs: 0.301 / % possible all: 55.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1Q25 Resolution: 2.2→29.96 Å / Rfactor Rfree error: 0.006 / Occupancy max: 1 / Occupancy min: 1 / Cross valid method: THROUGHOUT / σ(F): 1 / Stereochemistry target values: ENGH & HUBER
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Solvent computation | Solvent model: CNS BULK SOLVENT MODEL USED / Bsol: 41.38 Å2 / ksol: 0.33 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 39.96 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.2→29.96 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.3 Å / Rfactor Rfree error: 0.026 / Total num. of bins used: 8
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