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- PDB-1sqj: Crystal Structure Analysis of Oligoxyloglucan reducing-end-specif... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1sqj | ||||||
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Title | Crystal Structure Analysis of Oligoxyloglucan reducing-end-specific cellobiohydrolase (OXG-RCBH) | ||||||
![]() | oligoxyloglucan reducing-end-specific cellobiohydrolase![]() | ||||||
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Function / homology | ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yaoi, K. / Kondo, H. / Noro, N. / Suzuki, M. / Tsuda, S. / Mitsuishi, Y. | ||||||
![]() | ![]() Title: Tandem Repeat of a Seven-Bladed beta-Propeller Domain in Oligoxyloglucan Reducing-End-Specific Cellobiohydrolase Authors: Yaoi, K. / Kondo, H. / Noro, N. / Suzuki, M. / Tsuda, S. / Mitsuishi, Y. #1: Journal: J.BIOL.CHEM. / Year: 2002 Title: Purification, characterization, cloning, and expression of a novel xyloglucan-specific glycosidase, oligoxyloglucan reducing end-specific cellobiohydrolase Authors: Yaoi, K. / Mitsuishi, Y. #2: Journal: ACTA CRYSTALLOGR.,SECT.D / Year: 2003 Title: Crystallization and preliminary X-ray crystallographic study on a xyloglucan-specific exo-beta-glycosidase, oligoxyloglucan reducing-end specific cellobiohydrolase Authors: Yaoi, K. / Kondo, H. / Suzuki, M. / Noro, N. / Tsuda, S. / Mitsuishi, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 286.8 KB | Display | ![]() |
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PDB format | ![]() | 240.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 84974.727 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q8J0D2, ![]() #2: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.79 Å3/Da / Density % sol: 55.9 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion / pH: 5.8 Details: PEG 3000, PEG 400, pH 5.8, VAPOR DIFFUSION, temperature 293K |
-Data collection
Diffraction | Mean temperature: 95 K | |||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Nov 19, 2002 / Details: mirrors | |||||||||||||||
Radiation | Monochromator: Si(111) / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.2→40 Å / Num. all: 294393 / Num. obs: 294393 / % possible obs: 87.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.5 % / Rmerge(I) obs: 0.069 / Net I/σ(I): 9.5 | |||||||||||||||
Reflection shell | Resolution: 2.2→2.32 Å / Redundancy: 3 % / Rmerge(I) obs: 0.252 / Mean I/σ(I) obs: 3 / % possible all: 70.9 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.686 Å2
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Refinement step | Cycle: LAST / Resolution: 2.2→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.256 Å / Total num. of bins used: 20
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