+Open data
-Basic information
Entry | Database: PDB / ID: 1rna | ||||||||||||||||||
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Title | CRYSTALLOGRAPHIC STRUCTURE OF AN RNA HELIX: [U(U-A)6A]2 | ||||||||||||||||||
Components | RNA (5'-R(*Keywords | RNA / A-RNA / DOUBLE HELIX | Function / homology | RNA / RNA (> 10) | Function and homology information Method | X-RAY DIFFRACTION / Resolution: 2.25 Å | Authors | Dock-Bregeon, A.C. / Chevrier, B. / Podjarny, A. / Johnson, J. / De Bear, J.S. / Gough, G.R. / Gilham, P.T. / Moras, D. | Citation | Journal: J.Mol.Biol. / Year: 1989 Title: Crystallographic structure of an RNA helix: [U(UA)6A]2. Authors: Dock-Bregeon, A.C. / Chevrier, B. / Podjarny, A. / Johnson, J. / de Bear, J.S. / Gough, G.R. / Gilham, P.T. / Moras, D. #1: Journal: Nature / Year: 1988 Title: High Resolution Structure of the RNA Duplex [u(u-A)6a]2 Authors: Dock-Bregeon, A.C. / Chevier, B. / Podjarny, A. / Moras, D. / De Bear, J.S. / Gough, G.R. / Gilham, P.T. / Johnson, J.E. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1rna.cif.gz | 26.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1rna.ent.gz | 17.3 KB | Display | PDB format |
PDBx/mmJSON format | 1rna.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rn/1rna ftp://data.pdbj.org/pub/pdb/validation_reports/rn/1rna | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: RNA chain | Mass: 4402.644 Da / Num. of mol.: 2 / Source method: obtained synthetically #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 42.03 % | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 308 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 308.00K | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Temperature: 35 ℃ / pH: 6.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 291 K |
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Detector | Type: ENRAF-NONIUS CAD4 / Detector: DIFFRACTOMETER |
Radiation | Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.25→25 Å / Num. all: 4924 / Num. obs: 2492 / Observed criterion σ(F): 2 |
Reflection | *PLUS Highest resolution: 2.25 Å / Lowest resolution: 25 Å / Num. obs: 4924 / Observed criterion σ(F): 2 |
-Processing
Software | Name: NUCLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 2.25→10 Å / Occupancy max: 1 / Occupancy min: 0.51 / σ(F): 2 /
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Refine Biso |
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Refinement step | Cycle: LAST / Resolution: 2.25→10 Å
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Refine LS restraints |
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Refinement | *PLUS σ(F): 2 / Highest resolution: 2.25 Å / Lowest resolution: 10 Å / Num. reflection obs: 2437 / Rfactor obs: 0.131 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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