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- PDB-1rmn: A THREE-DIMENSIONAL MODEL FOR THE HAMMERHEAD RIBOZYME BASED ON FL... -

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Basic information

Entry
Database: PDB / ID: 1rmn
TitleA THREE-DIMENSIONAL MODEL FOR THE HAMMERHEAD RIBOZYME BASED ON FLUORESCENCE MEASUREMENTS
ComponentsRNA (49-MER)
KeywordsRIBONUCLEIC ACID(HAMMERHEAD RIBOZYME)
Function / homologyRNA / RNA (> 10)
Function and homology information
MethodFLUORESCENCE TRANSFER
AuthorsTuschl, T. / Gohlke, C. / Jovin, T.M. / Westhof, E. / Eckstein, F.
CitationJournal: Science / Year: 1994
Title: A three-dimensional model for the hammerhead ribozyme based on fluorescence measurements.
Authors: Tuschl, T. / Gohlke, C. / Jovin, T.M. / Westhof, E. / Eckstein, F.
History
DepositionOct 20, 1994Processing site: BNL
Revision 1.0Jan 26, 1995Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 14, 2024Group: Data collection / Database references / Other
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: RNA (49-MER)


Theoretical massNumber of molelcules
Total (without water)15,8591
Polymers15,8591
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

#1: RNA chain RNA (49-MER)


Mass: 15858.540 Da / Num. of mol.: 1 / Source method: obtained synthetically

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Experimental details

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Experiment

ExperimentMethod: FLUORESCENCE TRANSFER

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Processing

Software
NameClassification
NUCLIN/NUCLSQrefinement
NUCLINrefinement
NUCLSQrefinement
RefinementDetails: FLUORESCENCE RESONANCE ENERGY TRANSFER (FRET) WAS USED TO STUDY THE STRUCTURE OF THE RIBOZYME IN SOLUTION IN ORDER TO ESTABLISH THE RELATIVE SPATIAL ORIENTATION OF THE THREE CONSTITUENT ...Details: FLUORESCENCE RESONANCE ENERGY TRANSFER (FRET) WAS USED TO STUDY THE STRUCTURE OF THE RIBOZYME IN SOLUTION IN ORDER TO ESTABLISH THE RELATIVE SPATIAL ORIENTATION OF THE THREE CONSTITUENT WATSON-CRICK BASE-PAIRED HELICAL SEGMENTS.

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